首页|Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides

Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides

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Given the limited studies and conflicting findings,the transport character of ginsenosides crossing the blood-brain barrier (BBB) remains unclear.The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins,using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry.In brain tissue homogenates,ginsenoside Rgl was detectable and no other ginsenosides or their metabolites were found.No ginsenosides were detected in cerebrospinal fluid.Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues.Furthermore,immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes,and in few neurons.These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB,although the transport capability of ginsenosides through the BBB may be poor.

GinsengGinsenosideImmunostainingMass spectrometryBlood-brain barrier

ZHAO Yu-Nan、SHAO Xiao、OUYANG Liu-Feng、CHEN Lin、GU Ling

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Laboratory of Pathological Sciences, College of Medicine and Life, Nanjing University of Chinese Medicine, Nanjing 210046,China

Laboratory of Pathological Sciences, College of Medicine, Yan'an University, Yan'an 716000, China

Research Center, Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, China

work was supported by the National Natural Science Foundation of Chinawork was supported by the National Natural Science Foundation of ChinaProjects of Priority-Discipline for Colleges and Universities of Jiangsu Province (integration of traditional Chinese and wester

8130324681703732

2018

10.3724/SP.J.1009.2018.00175

中国天然药物
中国药科大学,中国药学会

中国天然药物

CSTPCDCSCD
影响因子：0.859
ISSN：1672-3651
年,卷(期)：2018.16(3)
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