Abstract
Xiaoaiping(XAP)Injection demonstrates the anti-prostate cancer(PCa)effects,yet the under-lying mechanism remains unclear.This study aims to investigate the impact of XAP on PCa and elucidate its mechanism of action.PCa cell proliferation was evaluated using a cell count-ing kit-8(CCK-8)assay.Cell apoptosis was assessed through Hoechst staining and Western blotting assays.Proteomics technology was employed to identify key molecules and signific-ant signaling pathways modulated by XAP in PCa cells.To further validate potential key genes and important pathways,a series of assays were conducted,including acridine orange(AO)staining,transmission electron microscopy,and immunofluorescence assays.The molecular mechanism of XAP against PCa in vivo was examined using a PC3 xenograft mouse model.Res-ults demonstrated that XAP significantly inhibited cell proliferation in multiple PCa cell lines.In C4-2 and prostate cancer cell line-3(PC3)cells,XAP induced cellular apoptosis,evidenced by reduced B-cell lymphoma 2(Bcl-2)levels and elevated Bcl-2-associated X(Bax)levels.Pro-teomic,immunofluorescence,and quantitative reverse transcription-polymerase chain reac-tion(qRT-PCR)investigations revealed a strong correlation between forkhead box O3a(FoxO3a)autophagic degradation and the anti-PCa action of XAP.XAP hindered autophagy by reducing the expression levels of autophagy-related protein 5(Atg5)/autophagy-related pro-tein 12(Atg12)and enhancing FoxO3a expression and nuclear translocation.Furthermore,XAP exhibited potent anti-PCa action in PC3 xenograft mice and triggered FoxO3a nuclear translocation in tumor tissue.These findings suggest that XAP induces PCa apoptosis via in-hibition of FoxO3a autophagic degradation,potentially offering a novel perspective on XAP in-jection as an effective anticancer therapy for PCa.
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