首页|Buqi-Tongluo Decoction inhibits osteoclastogenesis and alleviates bone loss in ovariectomized rats by attenuating NFATc1,MAPK,NF-κB sig-naling

Buqi-Tongluo Decoction inhibits osteoclastogenesis and alleviates bone loss in ovariectomized rats by attenuating NFATc1,MAPK,NF-κB sig-naling

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Osteoporosis is a prevalent skeletal condition characterized by reduced bone mass and strength,leading to increased fragility.Buqi-Tongluo(BQTL)decoction,a traditional Chinese medicine(TCM)prescription,has yet to be fully evaluated for its potential in treating bone diseases such as osteoporosis.To investigate the mechanism by which BQTL decoction inhib-its osteoclast differentiation in vitro and validate these findings through in vivo experiments.We employed MTS assays to assess the potential proliferative or toxic effects of BQTL on bone marrow macrophages(BMMs)at various concentrations.TRAcP experiments were conduc-ted to examine BQTL's impact on osteoclast differentiation.RT-PCR and Western blot ana-lyses were utilized to evaluate the relative expression levels of osteoclast-specific genes and proteins under BQTL stimulation.Finally,in vivo experiments were performed using an osteo-porosis model to further validate the in vitro findings.This study revealed that BQTL sup-pressed receptor activator of NF-κB ligand(RANKL)-induced osteoclastogenesis and osteo-clast resorption activity in vitro in a dose-dependent manner without observable cytotoxicity.The inhibitory effects of BQTL on osteoclast formation and function were attributed to the downregulation of NFATc1 and c-fos activity,primarily through attenuation of the MAPK,NF-κB,and Calcineurin signaling pathways.BQTL's inhibitory capacity was further examined in vivo using an ovariectomized(OVX)rat model,demonstrating a strong protective effect against bone loss.BQTL may serve as an effective therapeutic TCM for the treatment of post-menopausal osteoporosis and the alleviation of bone loss induced by estrogen deficiency and related conditions.

OsteoporosisEstrogen deficiencyOsteoclastBuqi-TongluoNFATc1MAPKNF-κB

Yongxian Li、Jinbo Yuan、Wei Deng、Haishan Li、Yuewei Lin、Jiamin Yang、Kai Chen、Heng Qiu、Ziyi Wang、Vincent Kuek、Dongping Wang、Zhen Zhang、Bin Mai、Yang Shao、Pan Kang、Qiuli Qin、Jinglan Li、Huizhi Guo、Yanhuai Ma、Danqing Guo、Guoye Mo、Yijing Fang、Renxiang Tan、Chenguang Zhan、Teng Liu、Guoning Gu、Kai Yuan、Yongchao Tang、De Liang、Liangliang Xu、Jiake Xu、Shuncong Zhang

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The First Clinical Academy,Guangzhou University of Chinese Medicine,Guangzhou 510405,China

The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510407,China

Lingnan Medical Research Center of Guangzhou University of Chinese Medicine,Guangzhou 510405,China

School of Biomedical Sciences,University of Western Australia,Western Australia 6102,Australia

Curtin Medical School,Curtin University,Western Australia 6102,Australia

Foshan Hospital of Traditional Chinese Medicine,Foshan 528000,China

School of Public Health and Management,Guangzhou University of Chinese Medicine,Guangzhou 510405,China

The State Key Laboratory of Pharmaceutical Biotechnology,Institute of Functional Biomolecules,Nanjing University,Nanjing 210008,China

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2025

10.1016/S1875-5364(25)60810-7

中国天然药物
中国药科大学,中国药学会

中国天然药物

影响因子：0.859
ISSN：1672-3651
年,卷(期)：2025.23(1)