醋酸菌的分离提纯与诱变育种
Separation and Purification of Acetic Acid Bacteria and Its Mutation Breeding
彭桂兰 1孙祖莉1
作者信息
摘要
实验以研究陕西农家醋醅、自制果醋的醋酸菌为出发点,采用碳酸钙培养基透明圈法分离醋酸菌.将6个HC值较大的单菌落接入平板分离纯化,经革兰氏染色及产醋酸定性试验,最后得到了A,B2株醋酸菌.将这2株菌经紫外诱变,产酸量分别提高18％和4.9％；经甲基磺酸乙酯(EMS)诱变,产酸量分剐得到提高.
Abstract
Take acetic acid bacteria from Shaanxi farmhouse brewing mass and homemade fruit vinegar as bacterial strain,separate acetic acid bacteria with calcium carbonate culture medium transparent circle method.Six single colonies with larger HC value are separated and purified by plate culture.The A and B strains of acetic acid bacteria are obtained by gram staining and producing acetic acid qualitative test.The acid yield by the two strains with ultraviolet mutation increases by 18％ and 4.9％.The acid yield by ethyl methyl sulfonate (EMS) mutation increases.
关键词
醋酸菌/分离/紫外诱变/甲基磺酸乙酯(EMS)诱变Key words
acetic acid bacteria/separation/ultraviolet (UV) mutagenesis/ethyl methyl sulfonate (EMS) mutagenesis引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据