Study on Optimization of Culture Medium for Lipase Production by Pseudomonas sp.HFE733 Using Response Surface Methodology
In this study,response surface methodology (RSM) is employed to optimize the fermentation culture medium for lipase production by Pseudomonas sp.HFE733 in submerged fermentation.Based on single factor experiments,two significant influence factors are screened by the method of Plackett-Burman design as:yeast extract and olive oil.To reduce the production cost,the minimum additive amount experiment is used to find out the minimum additive amount of non-significant factors,and the steepest ascent design is applied to obtain the center point of central composite design,and the optimal concentration and correlations of two factors are identified by central composite design.The result indicates that the optimal fermentation components for lipase production are as follows:yeast extract is 34 g/L,olive oil is 5.92 mL/L,sucrose is 12.5 g/L,CuSO4 · 5H2O is 0.4 g/L,MnSO4 · H2O is 0.2 g/L.The enzyme activity in verification test reaches 9.27 U/mL,consistent with the model predicted value,which is 104.64％ higher than that of single factor experiments' results (4.53 U/mL).
Pseudomonas sp.lipaseRSMculture medium optimization
NETL
NSTL
万方数据
维普
