Analysis of Ultrasonic-Assisted Extraction Process and Antibacterial Effect of Osmanthus fragrans Pigment
Osmanthus fragrans pigment is a kind of natural pigment with excellent performance,which has broad application prospect in the food processing industry.In this test,with Pucheng Osmanthus fragrans as the raw material,the effects of Osmanthus fragrans varieties,ethanol concentration,ultrasonic power,ultrasonic time and liquid-solid ratio on the yield of Osmanthus fragrans pigment are investigated by single factor test,and the extraction process is further optimized by response surface test.At the same time,Oxford cup method is used to verify the antibacterial effects of Osmanthus fragrans pigment on seven strains(including four strains of bacteria,two strains of filamentous fungi and one strain of yeast).The results show that the optimal extraction conditions of Osmanthus fragrans pigment by ultrasonic-assisted extraction method are ethanol concentration of 60％,liquid-solid ratio of 25∶1(mL/g),ultrasonic power of 200 W and ultrasonic time of 30 min.Under these conditions,the yield of Osmanthus fragrans pigment is the highest,reaching 61.56％.In addition,in the antibacterial test,it is confirmed that the Osmanthus fragrans pigment has significant inhibitory effects on Bacillus subtilis,Staphylococcus aureus,Escherichia coli,Micrococcus luteus and Saccharomyces cerevisiae,while the inhibitory effects on Penicillium citrinum and Aspergillus oryzae are relatively weaker.
NETL
NSTL
万方数据
维普
