响应面法优化栗蘑多糖提取工艺及其抗氧化活性研究
Optimization of Extraction Process of Polysaccharides from Grifola frondosa by Response Surface Methodology and Study on Their Antioxidant Activity
牛牧青 1徐婉婷 1龙丽芳 2吕锐玲 2徐碧林 2郑永良2
作者信息
- 1. 黄冈师范学院生物与农业资源学院,湖北黄冈 438000;华中农业大学生命科学技术学院,武汉 430070
- 2. 黄冈师范学院生物与农业资源学院,湖北黄冈 438000;经济林木种质改良及资源综合利用湖北省重点实验室,湖北黄冈 438000
- 折叠
摘要
为优化栗蘑多糖提取工艺,以栗蘑子实体为研究对象,通过硫酸-苯酚滴定法,结合紫外分光光度法测定栗蘑多糖提取率.在单因素多水平实验的基础上,采用响应面法,利用Design-Expert软件,选取提取时间、提取温度、提取液料比3个因素,设置三水平实验方案优化提取工艺.结果表明,栗蘑多糖最佳提取工艺为提取时间1.9 h、提取温度80 ℃、提取液料比37∶1(mL/g),此条件下栗蘑多糖提取率为14.45％.抗氧化活性研究结果表明,栗蘑多糖对DPPH自由基和羟基自由基都具有较好的清除能力,在一定范围内,抗氧化活性随着多糖浓度的增加而提高.
Abstract
In order to optimize the extraction process of Grifola frondosa polysaccharides,with Grifola frondosa fruit body as the research object,the extraction rate of polysaccharides from Grifola frondosa is determined by sulfuric acid-phenol titration method combined with ultraviolet spectrophotometry.On the basis of single-factor and multi-level experiment,response surface methodology and Design-Expert software are used to select three factors such as extraction time,extraction temperature and extraction liquid-solid ratio.A three-level experimental scheme is set up to optimize the extraction process.The results show that the optimum extraction conditions are as follows:extraction time is 1.9 h,extraction temperature is 80 ℃,extraction liquid-solid ratio is 37∶1(mL/g),under such conditions,the extraction rate of polysaccharides is 14.45％.Antioxidant activity research results show that the polysaccharides from Grifola frondosa have good scavenging capacity on DPPH free radical and hydroxyl free radical.Within a certain range,the antioxidant activity increases with the increase of polysaccharides concentration.
关键词
栗蘑/多糖/响应面法/提取工艺/优化/抗氧化活性Key words
Grifola frondosa/polysaccharides/response surface methodology/extraction process/optimization/antioxidant activity引用本文复制引用
基金项目
国家自然科学基金青年项目(32001207)
湖北省中央引导地方科技发展专项资金(2019zyyd044)
湖北省重点研发计划项目(2020BBB091)
出版年
2024
NETL
NSTL
维普
万方数据