Process Optimization and Characterization of Gelatin Composite Film Modified by TGase Associated with k-Carrageenan
In order to develop a natural preservative film with excellent performance,in this study,with gelatin as the substrate,k-carrageenan and transglutaminase(TGase)are used to modify gelatin,and a new gelatin composite film is prepared by tape casting method.The effects of the addition amount of k-carrageenan,the addition amount of TGase,the reaction time of TGase and the drying temperature on the physical properties of gelatin composite film are investigated by single factor test and orthogonal test.It is determined that the optimum preparation process is 5％gelatin,0.5％k-carrageenan,0.2％TGase,reaction time 60 min and drying temperature 45 ℃.The tensile strength of the gelatin composite film prepared under the optimum process conditions is 14.33 MPa,the elongation of break is 44.24％,the water solubility is 26.72％and the water vapor permeability coefficient is 0.278 g·mm/(kPa·h·m2).After the optimization of preparation process,the structures of different gelatin composite films are characterized by X-ray diffraction and thermogravimetric analysis.The characterization results show that k-carrageenan and TGase synergistically modify gelatin through electrostatic interaction and amino acid isopeptide bonds respectively.This study has provided a theoretical basis for the development of new gelatin composite film.
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