Double-Enzyme Stepwise Enzymatic Hydrolysis Process of Yanjin Silky Fowl Chicken and Its Amino Acid Composition Analysis
With Yunnan Yanjin silky fowl chicken as the raw material,protein hydrolysis degree as the index,the enzymatic hydrolysis conditions of Yanjin silky fowl chicken is optimized by response surface analysis method.The amino acid composition and molecular weight distribution of polypeptides of hydrolysates are detected by amino acid analyzer and gel permeation chromatography.The results show that the optimal process conditions for double-enzyme stepwise enzymatic hydrolysis are as follows:the enzymatic hydrolysis time of animal protease is 5 h,the addition amount of enzyme is 2.6％,the pH is 6.5,after enzyme inactivation,papain is added for 3 h enzymatic hydrolysis,the enzyme addition amount is 2.5％,and the enzymatic hydrolysis temperature is 60 ℃.Under such conditions,the hydrolysis degree of Yanjin silky fowl chicken is(32.62±0.79)％,there are 17 amino acids in the enzymatic hydrolysate,the content of total free amino acids could reach 6.02 mg/mL,the content of flavor amino acids such as leucine and arginine is higher,and the relative molecular weight of polypeptides is mainly concentrated below 1 kDa.The research results could provide some theoretical basis for the development of new seasoning products of Yanjin silky fowl chicken in the future.
万方数据
维普
