中国调味品2024,Vol.49Issue(2) :62-68,78.DOI:10.3969/j.issn.1000-9973.2024.02.009

高产色素低产桔青霉素红曲霉菌筛选及发酵工艺优化研究

Study on Screening and Fermentation Process Optimization of Monascus with High-Yield Pigment and Low-Yield Citrinin

殷倩倩 左勇
中国调味品2024,Vol.49Issue(2) :62-68,78.DOI:10.3969/j.issn.1000-9973.2024.02.009

高产色素低产桔青霉素红曲霉菌筛选及发酵工艺优化研究

Study on Screening and Fermentation Process Optimization of Monascus with High-Yield Pigment and Low-Yield Citrinin

殷倩倩 1左勇1
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作者信息

  • 1. 四川师范大学 生命科学学院,成都 610101
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摘要

该研究通过扩大菌株分离源,选育优质红曲霉菌M7-5.以该菌株为发酵菌株,采用单因素试验研究大米糊化水量、发酵温度、碳源种类及添加量、氮源种类及添加量对红曲发酵色价及桔青霉素含量的影响,在此基础上,利用响应面试验优化最佳控制参数.结果表明,红曲最佳发酵条件为大米糊化水量13%、甘油添加量8%、硝酸钠添加量1.85%,在此条件下,色价与桔青霉素含量的比值(p/c值)达到22 158.2.该研究可为传统红曲发酵工艺的改进和工业化应用提供参考.

Abstract

In this study,high-quality Monascus M7-5 is selected by expanding the source of strain isolation.Using this strain as the fermentation strain,the effects of rice gelatinization water amount,fermentation temperature,carbon source type and addition amount,nitrogen source type and addition amount on the color value of Monascus fermentation and citrinin content are studied by single factor test.On this basis,response surface test is used to optimize the optimal control parameters.The results show that the optimum fermentation conditions of Monascus are as follows:the rice gelatinization water amount is 13%,glycerin addition amount is 8%and sodium nitrate addition amount is 1.85%.Under these conditions,the ratio of color value to citrinin content(p/c value)reaches 22 158.2.This study can provide references for the improvement of traditional Monascus fermentation process and industrial application.

关键词

红曲/发酵工艺/色价/桔青霉素/响应面

Key words

Monascus/fermentation process/color value/citrinin/response surface

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基金项目

中央引导地方-区域创新体系建设项目(2021ZYD0159)

四川省科技计划重点研发项目(2022YFN0056)

四川省科技厅应用基础研究项目(2021YJ0273)

出版年

2024
中国调味品
全国调味品科技情报中心站

中国调味品

CSTPCD北大核心
影响因子:0.541
ISSN:1000-9973
被引量1
参考文献量13
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