双酶辅助水蒸气蒸馏法提取肉豆蔻精油及其GC-MS成分分析

Extraction of Myristica fragrans Houtt.Essential Oil by Dual Enzyme Assisted Steam Distillation and Its Component Analysis by GC-MS

盖旭 ¹程庠 ²姜子涛 ³罗君萍¹

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作者信息

1. 天津天狮学院食品工程学院,天津 301700
2. 天津中检海吉星食品检测有限公司,天津 301600
3. 天津天狮学院食品工程学院,天津 301700;天津商业大学生物技术与食品科学学院,天津 300134
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摘要

采用双酶辅助水蒸气蒸馏法提取广西产肉豆蔻的精油,并通过GC-MS对其进行成分分析.正交实验结果表明,最优提取工艺条件为加酶量2％、酶解pH 4.0、酶解温度40 ℃,在此条件下,肉豆蔻精油的得率为6.50 mL/100 g.利用GC-MS和保留指数法从精油中鉴定出56种化合物,占精油总量的99.71％,主要成分为肉豆蔻醚(16.60％)、甲基丁香酚(14.78％)、桧烯(13.47％)、(+)-柠檬烯(7.29％)、4-萜烯醇(6.75％)、榄香素(6.62％)、γ-松油烯(4.28％)、反式-甲基异丁香酚(3.73％)、β-蒎烯(3.59％)和黄樟素(3.22％).

Abstract

Dual enzyme assisted steam distillation is used to extract essential oil from Myristica fragrans Houtt.in Guangxi,and its components are analyzed by GC-MS.The orthogonal experimental results indicate that the optimal extraction process conditions are enzyme addition amount of 2％,enzymatic hydrolysis pH of 4.0 and enzymatic hydrolysis temperature of 40 ℃.Under these conditions,the yield of essential oil from Myristica fragrans Houtt.is 6.50 mL/100 g.Fifty-six compounds are identified in essential oil by GC-MS and retention index method,accounting for 99.71％of the total essential oil.The main components are myristicin(16.60％),methyleugenol(14.78％),sabinene(13.47％),(+)-limonene(7.29％),terpinen-4-ol(6.75％),elemicin(6.62％),γ-terpinene(4.28％),trans-methyl eugenol(3.73％),β-pinene(3.59％)and safrole(3.22％).

关键词

肉豆蔻精油/双酶辅助水蒸气蒸馏/提取/气相色谱-质谱/成分分析/保留指数

Key words

essential oil from Myristica fragrans Houtt./dual enzyme assisted steam distillation/extraction/GC-MS/component analysis/retention index

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基金项目

天津市教委科研计划项目(2023KJ287)

出版年

2024

中国调味品

全国调味品科技情报中心站

中国调味品

CSTPCD北大核心

影响因子：0.541

ISSN：1000-9973

参考文献量14

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