Extraction of Myristica fragrans Houtt.Essential Oil by Dual Enzyme Assisted Steam Distillation and Its Component Analysis by GC-MS
Dual enzyme assisted steam distillation is used to extract essential oil from Myristica fragrans Houtt.in Guangxi,and its components are analyzed by GC-MS.The orthogonal experimental results indicate that the optimal extraction process conditions are enzyme addition amount of 2％,enzymatic hydrolysis pH of 4.0 and enzymatic hydrolysis temperature of 40 ℃.Under these conditions,the yield of essential oil from Myristica fragrans Houtt.is 6.50 mL/100 g.Fifty-six compounds are identified in essential oil by GC-MS and retention index method,accounting for 99.71％of the total essential oil.The main components are myristicin(16.60％),methyleugenol(14.78％),sabinene(13.47％),(+)-limonene(7.29％),terpinen-4-ol(6.75％),elemicin(6.62％),γ-terpinene(4.28％),trans-methyl eugenol(3.73％),β-pinene(3.59％)and safrole(3.22％).
essential oil from Myristica fragrans Houtt.dual enzyme assisted steam distillationextractionGC-MScomponent analysisretention index
万方数据
维普
