Study on Process of Efficient Synthesis of Vanillic Acid Using Deep Eutectic Solvent-Assisted Enzymatic Method
In response to the issues of environmental contamination and low activity caused by chemical synthesis of vanillic acid,as well as the low yield and high production cost of extracting vanillic acid from plants,vanillin dehydrogenase derived from Bacillus ligniniphilus L1 is used for the biotransformation of vanillic acid.In order to better improve the efficiency of enzymatic catalysis reaction,adding an appropriate amount of deep eutectic solvent(DES,choline chloride:1,4-butanediol is 1∶3)into the reaction system can effectively improve the enzyme activity and make it maintain high stability,and then the effects of reaction temperature,reaction pH,reaction time and the addition amount of DES on the conversion rate of vanillic acid are investigated.Response surface experiment is applied to study the process,and the optimal transformation process parameters are determined as follows:reaction temperature is 36 ℃,reaction pH is 6.8 and DES addition amount is 17％.Under these conditions,the conversion rate of vanillic acid reaches 84.57％,which is 37.98％higher than that of vanillic acid without adding DES.The use of DES-assisted enzymatic method for the biotransformation of vanillic acid can effectively improve the enzyme activity of vanillin dehydrogenase and the conversion rate of vanillic acid.The preparation process is green,safe and economical,which meets the requirements for green industrial production of vanillic acid.
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