Optimization of Compound Enzymatic Hydrolysis Process of Euryale ferox Powder by Response Surface Methodology and Study on Antioxidant Activity of Polysaccharides
To determine the optimal compound enzymatic hydrolysis process of Euryale ferox powder and the antioxidant activity of Euryale ferox polysaccharides,with Euryale ferox powder from high-quality places of origin as the raw material,the effects of the addition amount of compound enzymes,pH,enzymatic hydrolysis temperature and enzymatic hydrolysis time on the hydrolysis degree(dextrose equivalent,DE value)are studied through single factor test,Box-Behnken test design and response surface analysis.After enzymatic hydrolysis,the antioxidant activity of Euryale ferox polysaccharides is evaluated by measuring their DPPH free radical scavenging capacity.The results show that the optimal process parameters for enzymatic hydrolysis of Euryale ferox powder using compound enzymes method are as follows:the mass ratio of compound enzymes(pullulanase:α-amylase)is 5∶6,the addition amount of enzymes is 0.3％(based on the mass of raw materials),pH is 6.5,enzymatic hydrolysis temperature is 56 ℃ and enzymatic hydrolysis time is 45 min.Under these conditions,the hydrolysis degree of Euryale ferox powder basic material can reach 20.25％,and after enzymatic hydrolysis,Euryale ferox polysaccharides have certain antioxidant activity.The research results have provided theoretical and methodological basis for the development and utilization of products related to Euryale ferox.
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