Study on Purification Process of Soybean Isoflavones from Sauce Residue by Macroporous Resin and Its Antioxidant Activity
唐银 1陈婧司 2沈子绮 1何贵萍 1张佳琪 1吕远平1
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作者信息
1. 四川大学轻工科学与工程学院,成都 610065
2. 苏州大学苏州医学院公共卫生学院,江苏苏州 215123
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摘要
研究酱渣大豆异黄酮的纯化工艺及其抗氧化活性.选用5种树脂(AB-8、HPD300、ADS-7、DM301、D101)进行静态吸附试验,筛选吸附效果最好的树脂,再以动态吸附试验筛选最佳纯化工艺,最后研究纯化后酱渣大豆异黄酮的抗氧化活性.结果表明,AB-8树脂的吸附效果最好,在流速1.5 mL/min、pH 3的条件下吸附0.6 mg/mL的样品溶液75 mL,再用100 mL 60％乙醇溶液解吸,样品纯度可由11.37％提升到55.84％;纯化后的酱渣大豆异黄酮具有较好的抗氧化活性,其浓度为0.07 mg/mL时,对DPPH自由基和ABTS自由基的清除率分别为93.35％和98.67％.AB-8树脂可用于酱渣大豆异黄酮的分离纯化且效果较好,酱渣大豆异黄酮具有较强的抗氧化活性.
Abstract
The purification process and antioxidant activity of soybean isoflavones from sauce residue are studied.Five resins(AB-8,HPD300,ADS-7,DM301 and D101)are selected for static adsorption test to screen the resin with the best adsorption effect,and then the optimal purification process is screened by dynamic adsorption test.Finally,the antioxidant activity of soybean isoflavones from sauce residue after purification is studied.The results show that AB-8 resin has the best adsorption effect.Under the conditions of the flow rate of 1.5 mL/min and pH 3,75 mL 0.6 mg/mL sample solution is adsorbed,and then desorbed with 100 mL 60％ethanol solution.The purity of the sample can increase from 11.37％to 55.84％.The purified soybean isoflavones from sauce residue have good antioxidant activity.When its concentration is 0.07 mg/mL,the scavenging rates on DPPH radicals and ABTS radicals are 93.35％and 98.67％respectively.AB-8 resin can be used for the separation and purification of soybean isoflavones from sauce residue with good effect,and soybean isoflavones from sauce residue have strong antioxidant activity.
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