Analysis of Components and Biological Activity of Different Extracts from Hawthorn
Hawthorn is rich in fructose,glucose,fatty substances,protein,crude fiber,carotene and so on.It has biological activities such as anti-inflammatory,regulating blood sugar level and reducing blood pressure,and is widely used in food additives,condiments,health care products and so on.There are various methods for extracting active components from hawthorn,and the composition and content of compounds obtained by different extraction methods are different.At present,solvent extraction method is the most common extraction method.This method requires low technical requirements and is easy to operate,but there are obvious differences in the composition and content of compounds obtained by different solvents.In this experiment,the content of polyphenols,flavonoids and other active components in hawthorn extracts of six different solvents(methanol,ethanol,acetone,ethyl acetate,chloroform and petroleum ether)is determined by high performance liquid chromatography.The results show that the content of polyphenols and flavonoids in hawthorn methanol extract is the highest,and the content of polyphenols and flavonoids in hawthorn petroleum ether extract is the lowest;there are 10 active components identified in hawthorn extracts of six different solvents,which are catechin,chlorogenic acid,proanthocyanidin B2,epicatechin,quercetin,maslinic acid,hyperoside,isoquercitrin,caffeic acid and luteolin glucoside.The antioxidant and reducing properties of hawthorn compounds extracted by different solvents are analyzed.The results show that with the increase of the concentration of hawthorn extracts,the antioxidant and reducing properties increase,and the antioxidant and reducing properties of hawthorn methanol extract are stronger than those of other solvents.