中国调味品2024,Vol.49Issue(4) :11-17,24.DOI:10.3969/j.issn.1000-9973.2024.04.002

番茄酵素发酵工艺优化及营养成分变化分析

Optimization of Tomato Enzyme Fermentation Process and Analysis of Change of Nutritional Components

王瑞 陈洁如 刘亚平 李泽珍 孙胜 狄建兵
中国调味品2024,Vol.49Issue(4) :11-17,24.DOI:10.3969/j.issn.1000-9973.2024.04.002

番茄酵素发酵工艺优化及营养成分变化分析

Optimization of Tomato Enzyme Fermentation Process and Analysis of Change of Nutritional Components

王瑞 1陈洁如 1刘亚平 1李泽珍 1孙胜 2狄建兵1
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作者信息

  • 1. 山西农业大学食品科学与工程学院,山西晋中 030801
  • 2. 山西农业大学园艺学院,山西晋中 030801
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摘要

以番茄为原料,植物乳杆菌为发酵菌种,超氧化物歧化酶(SOD)和总酸为评价指标,采用单因素试验和Box-Behnken试验优化番茄酵素的发酵工艺,并分析监测番茄酵素发酵过程中营养成分和相关酶活性变化.结果表明,在最佳接种量0.3%、发酵温度37 ℃、初始pH值5.5、发酵时间40 h的条件下,番茄酵素SOD活性达147.63 U/g,总酸含量为48.60 g/kg.发酵过程中,pH值和可溶性固形物含量均随着发酵时间的增加而持续下降,总酸含量持续上升,还原糖、总酚含量及SOD活性、脂肪酶活性均先上升后下降,番茄红素含量先持续下降后上升再下降;同时对各营养成分与酶活性进行相关性分析,结果表明各营养成分之间存在一定相关性.

Abstract

With tomato as the raw material,Lactobacillus plantarum as the fermentation strain,superoxide dismutase(SOD)and total acid as the evaluation indexes,single factor test and Box-Behnken test are used to optimize the fermentation process of tomato enzyme,and the changes of nutritional components and related enzyme activity during the fermentation of tomato enzyme are analyzed and monitored.The results show that under the conditions of the optimum inoculation amount of 0.3%,fermentation temperature of 37 ℃,initial pH value of 5.5 and fermentation time of 40 h,SOD activity of tomato enzyme reaches 147.63 U/g,and the total acid content is 48.60 g/kg.During the fermentation,both pH value and content of soluble solids decrease continuously with the increase of fermentation time,while the total acid content increases continuously.The content of reducing sugar and total phenols,the activity of SOD and lipase all increase firstly and then decrease,while the content of lycopene firstly decreases continuously,and then increases and then decreases again.At the same time,correlation analysis is conducted on various nutritional components and enzyme activity,and the results show that there is a certain correlation among various nutritional components.

关键词

番茄酵素/发酵工艺/优化/营养成分

Key words

tomato enzyme/fermentation process/optimization/nutritional components

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基金项目

山西省重点研发课题(202102140601015-4)

出版年

2024
中国调味品
全国调味品科技情报中心站

中国调味品

CSTPCD北大核心
影响因子:0.541
ISSN:1000-9973
参考文献量27
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