Optimization of Extraction Process of Total Flavonoids from Allium mongolicum Regel and Study on Their Bioactivity
Allium mongolicum Regel is widely cultivated in the alpine region in China and is rich in amino acids,trace elements and various bioactive substances.Flavonoids are a kind of natural active components.Compared with the study on the extraction process of flavonoids from seasonings such as ginger and Amomum tsao-ko,the study on the extraction process of total flavonoids from Allium mongolicum Regel is relatively late.In order to fully research and develop the active components in Allium mongolicum Regel,the extraction process of total flavonoids from Allium mongolicum Regel is studied and optimized through single factor test and orthogonal test.The results show that the optimal extraction process of total flavonoids from Allium mongolicum Regel is solid-liquid ratio of 1∶30,extraction time of 15 min,ethanol concentration of 75％,extraction temperature of 90 ℃ and ultrasonic power of 70 W.At this time,the yield of total flavonoids from Allium mongolicum Regel is 13.241％.In addition,a comparative study is conducted on the reducing ability of the total flavonoids from Allium mongolicum Regel and BHT(2,6-di-tert-butyl-p-cresol).It is found that the total flavonoids from Allium mongolicum Regel has reducing ability,but the reducing ability is lower than that of BHT.
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