Analysis of Effect and Mechanism of Chinese Herbal Medicine on Nitrite Content in Fermented Pickles Based on High-Throughput Sequencing
In order to explore the effect and mechanism of two Chinese herbal medicines,namely honeysuckle and phellodendron,on nitrite content in fermented pickles,the microbial community structure of pickles is analyzed based on high-throughput sequencing technology,and spectrophotometry is used to analyze the nitrite content in pickles.The results show that the nitrite content of pickles added with 5％honeysuckle and 5％phellodendron(8.02,5.90 mg/kg)is significantly lower than that of the control group(12.87 mg/kg)(P＜0.05).Through high-throughput sequencing of pickle samples,a total of 202 operational taxonomic units(OTU)are obtained,belonging to 11 phyla,129 genera and 165 species.The Chao 1 index of microorganism in pickle samples added with 5％honeysuckle and 5％phellodendron fermented for 10 d is significantly lower than that of the control group(P＜0.05).Proteobacteria is the dominant phylum in each sample,with the relative content of 90％.The relative content of dominant microorganisms in genus level of microorganisms treated with different Chinese herbal medicines is quite different.The relative content of Pseudomonas in the control group,the pickle sample group added with honeysuckle and the pickle sample group added with phellodendron is 52％,1％1％respectively.The relative content of Serratia is 15％,0％,1％respectively.The relative content of Achromobacter is 7％,1％,0％respectively.The experimental results show that the inhibitory effect of honeysuckle and phellodendron on the formation of nitrite in fermented Chinese cabbage is due to their inhibition on the growth and reproduction of microorganisms of Enterobacteriaceae(Serratia,Achromobacter)and Pseudomonas.
NETL
NSTL
万方数据
维普
