Differential Analysis of Metabolites in Medicinal Diet Fish Soup Based on Non-Targeted Metabonomics
Medicinal diet fish soup is a delicacy with high nutritional value,but the research on its chemical composition characteristics is relatively few.Therefore,in this study,with fish soup and medicinal diet fish soup as the research objects,ultra-performance liquid chromatography-mass spectrometry(UPLC-MS/MS)metabonomics technology combined with principal component analysis(PCA),orthogonal partial least squares-discrimination analysis(OPLS-DA)and other multivariate statistical methods is used to investigate the differential metabolites in medicinal diet fish soup.Metabolites with significant differences are screened based on the criteria of variable importance in projection(VIP)＞1 and P＜0.05,and metabolic pathway analysis is carried out.The results show that a total of 20 categories and 176 differential metabolites are identified,including 35 amino acids and their derivatives,38 fatty acyls,25 organic acids and their derivatives,17 nucleotides and their derivatives,10 carbohydrates and their derivatives,and 18 flavonoid compounds.Through KEGG functional pathway enrichment analysis,six metabolic pathways are screened,including the biosynthesis of amino acids,the biosynthesis of valine,leucine and isoleucine,the biosynthesis of nucleotide sugars,ABC transporters,the metabolism of caffeine,and the metabolism of cysteine and methionine.Non-targeted metabonomics reveals the metabolic differences of medicinal diet fish soup at the overall level,which has provided a direction for its identification and quality evaluation.
medicinal diet fish soupnon-targeted metabolomicsdifferential metabolitespathway analysis
NETL
NSTL
万方数据
维普
