基于非靶向代谢组学的药膳鱼汤代谢产物差异分析
Differential Analysis of Metabolites in Medicinal Diet Fish Soup Based on Non-Targeted Metabonomics
王益娟 1周芳 2焦文娟 2赵甜甜 2林以琳 3张业辉 2南海军 4黄文5
作者信息
- 1. 广东药科大学 中药学院,广州 510006;广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广州 510610
- 2. 广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广州 510610
- 3. 华南理工大学食品科学与工程学院,广州 510641
- 4. 广东药科大学 中药学院,广州 510006
- 5. 广东省农业科学院水产研究所,广州 510645
- 折叠
摘要
药膳鱼汤是一道滋补价值高的佳肴,关于其化学组成特征的研究相对较少.因此,该研究以鱼汤、药膳鱼汤为研究对象,利用超高效液相色谱-质谱联用(ultra performance liquid chromatography-mass spectrometry,UPLC-MS/MS)代谢组学技术,结合主成分分析(principal components analysis,PCA)、正交偏最小二乘-判别分析(orthogonal partial least squares-discrimination analysis,OPLS-DA)等多元统计学方法,探究药膳鱼汤的差异代谢物.采用变量投影重要性VIP＞1且P＜0.05的标准,筛选差异显著的代谢物,并进行代谢通路分析.结果显示,共鉴定出20类176个差异代谢物,其中包括35个氨基酸及其衍生物,38个脂肪酰类,25个有机酸及其衍生物,17个核苷酸及其衍生物,10个碳水化合物及其衍生物,以及18个黄酮化合物.KEGG功能通路富集分析筛选出6条代谢通路,包括氨基酸的生物合成、缬氨酸、亮氨酸和异亮氨酸的生物合成、核苷酸糖的生物合成、ABC转运蛋白、咖啡因代谢以及半胱氨酸和蛋氨酸代谢.非靶向代谢组学从整体水平上揭示了药膳鱼汤的代谢差异,为其鉴别和品质评价提供了方向.
Abstract
Medicinal diet fish soup is a delicacy with high nutritional value,but the research on its chemical composition characteristics is relatively few.Therefore,in this study,with fish soup and medicinal diet fish soup as the research objects,ultra-performance liquid chromatography-mass spectrometry(UPLC-MS/MS)metabonomics technology combined with principal component analysis(PCA),orthogonal partial least squares-discrimination analysis(OPLS-DA)and other multivariate statistical methods is used to investigate the differential metabolites in medicinal diet fish soup.Metabolites with significant differences are screened based on the criteria of variable importance in projection(VIP)＞1 and P＜0.05,and metabolic pathway analysis is carried out.The results show that a total of 20 categories and 176 differential metabolites are identified,including 35 amino acids and their derivatives,38 fatty acyls,25 organic acids and their derivatives,17 nucleotides and their derivatives,10 carbohydrates and their derivatives,and 18 flavonoid compounds.Through KEGG functional pathway enrichment analysis,six metabolic pathways are screened,including the biosynthesis of amino acids,the biosynthesis of valine,leucine and isoleucine,the biosynthesis of nucleotide sugars,ABC transporters,the metabolism of caffeine,and the metabolism of cysteine and methionine.Non-targeted metabonomics reveals the metabolic differences of medicinal diet fish soup at the overall level,which has provided a direction for its identification and quality evaluation.
关键词
药膳鱼汤/非靶向代谢组学/差异代谢物/通路分析Key words
medicinal diet fish soup/non-targeted metabolomics/differential metabolites/pathway analysis引用本文复制引用
基金项目
广东省重点领域研发计划(2021B0202060001)
广东省农科院水产协同中心项目(XT202303)
佛山市市院合作水产预制菜研发中心项目(202401)
揭阳市科技大专项(2022DZX018)
出版年
2024
NETL
NSTL
维普
万方数据