首页|陈皮多糖提取工艺及其抗氧化活性研究

陈皮多糖提取工艺及其抗氧化活性研究

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该研究以广陈皮为原料,利用超声波辅助提取法提取陈皮多糖,采用单因素试验优化陈皮多糖提取工艺并对其抗氧化活性进行研究,建立以葡萄糖为标准品,采用苯酚-硫酸法测定广陈皮多糖含量的定量分析方法.采用正交试验以多糖得率为指标优化工艺参数:料液比为1∶45(g/mL),提取时间为10 min,超声功率为350 W,提取温度为65 ℃,在此条件下,陈皮多糖得率高达40.06%.结果表明,广陈皮多糖对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的最大清除率为85.19%,对羟基自由基(·OH)的最大清除率为63.42%.
Study on Extraction Technology and Antioxidant Activity of Polysaccharides from Tangerine Peel
In this study,with Citrus reticulata Blanco.as the raw material,the polysaccharides are extracted from tangerine peel by ultrasonic-assisted extraction method.Single factor test is used to optimize the extraction technology of polysaccharides from tangerine peel and study their antioxidant activities.A quantitative analytical method is established for the determination of polysaccharide content from tangerine peel by phenol-sulfuric acid method with glucose as the standard sample.The technology parameters are optimized by orthogonal test with the yield of polysaccharides as the index:the solid-liquid ratio is 1∶45(g/mL),the extraction time is 10 min,the ultrasonic power is 350 W and the extraction temperature is 65 ℃.Under these conditions,the yield of polysaccharides from tangerine peel could reach 40.06%.The results show that the maximum scavenging rate of polysaccharides to 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)and hydroxyl radical(·OH)is 85.19%and 63.42%respectively.

Citrus reticulata Blanco.polysaccharidesextraction technologyantioxidant activityscavenging rate

唐婷范、李雪松、卢梦影、田玉红、徐紫薇、程昊

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广西科技大学生物与化学工程学院,广西糖资源绿色加工重点实验室,广西柳州螺蛳粉工程技术研究中心,广西柳州 545006

广陈皮 多糖 提取工艺 抗氧化活性 清除率

国家自然科学基金广西自然科学基金面上项目广西自然科学基金面上项目

222680122022GXNSFAA0354902021GXNSFAA220067

2024

中国调味品
全国调味品科技情报中心站

中国调味品

CSTPCD北大核心
影响因子:0.541
ISSN:1000-9973
年,卷(期):2024.49(5)
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