Study on Extraction Technology and Antioxidant Activity of Polysaccharides from Tangerine Peel
In this study,with Citrus reticulata Blanco.as the raw material,the polysaccharides are extracted from tangerine peel by ultrasonic-assisted extraction method.Single factor test is used to optimize the extraction technology of polysaccharides from tangerine peel and study their antioxidant activities.A quantitative analytical method is established for the determination of polysaccharide content from tangerine peel by phenol-sulfuric acid method with glucose as the standard sample.The technology parameters are optimized by orthogonal test with the yield of polysaccharides as the index:the solid-liquid ratio is 1∶45(g/mL),the extraction time is 10 min,the ultrasonic power is 350 W and the extraction temperature is 65 ℃.Under these conditions,the yield of polysaccharides from tangerine peel could reach 40.06%.The results show that the maximum scavenging rate of polysaccharides to 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)and hydroxyl radical(·OH)is 85.19%and 63.42%respectively.