Extraction of Collagen from Jellyfish Using Enzyme-Ultrasound Assisted Extraction Method and Analysis of Its Composition
To determine the optimal process of enzyme-ultrasound assisted extraction of collagen from jellyfish,the process is optimized using single factor experiment and response surface experiment.The effects of pepsin addition amount,extraction time,ultrasound time and ultrasound power on the yield of jellyfish collagen are studied,and the composition of amino acids and structure of collagen are analyzed.The results show that the optimal conditions for enzyme-ultrasound assisted extraction of collagen from jellyfish are 1%pepsin,extraction time of 48 h,ultrasound time of 32 min and ultrasound power of 318 W,at this time,the yield of jellyfish collagen is 38.03%.Glycine is the main amino acid in jellyfish collagen,and the content is 24.96%.The UV absorption peak of jellyfish collagen is at 235 nm.There are typical infrared spectral characteristic absorption peaks of jellyfish collagen in amide A and B bands as well as Ⅰ,Ⅱ and Ⅲ bands.This study has provided a theoretical basis for the deep processing of jellyfish.
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