Study on Preparation Process of Zinc-Rich Oyster Peptides with Low Molecular Weight by Ultrasound-Assisted Enzymatic Hydrolysis Method
With Crassostrea hongkongensis as the raw material,the zinc-rich oyster enzymatic hydrolysate is prepared by enzymatic hydrolysis method,and its relative molecular weight chromatogram and amino acid composition are analyzed.Through protease screening and ultrasound pretreatment,single factor test,orthogonal test and time curves are used to optimize the enzymatic hydrolysis process,so as to improve the hydrolysis degree of enzymatic hydrolysate and the release amount of biological zinc.Using ultrasonic power of 500 W and ultrasonic time of 10 min for pretreatment,animal protease is selected for enzymatic hydrolysis.The optimal enzymatic hydrolysis process is as follows:the enzyme addition amount is 1.6%,enzymatic hydrolysis time is 4 h,enzymatic hydrolysis temperature is 50℃,solid-liquid ratio is 1∶4 and pH is 6.5.The hydrolysis degree of the obtained oyster enzymatic hydrolysate is 49.581%.The zinc content of the enzymatic hydrolysate(on dry basis)is 680.831 mg/kg.The components of oyster peptides with relative molecular weight less than 1 000 Da reach 81.87%,which are rich in acid amino acids,namely glutamic acid and aspartic acid.The enzymatic hydrolysate of Crassostrea hongkongensis contains abundant small-molecular peptides,with a relatively complete amino acid composition and high zinc content,which can lay a foundation for the development of zinc-rich products in the future.
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