超声辅助酶法制备富锌低分子牡蛎肽的工艺研究
Study on Preparation Process of Zinc-Rich Oyster Peptides with Low Molecular Weight by Ultrasound-Assisted Enzymatic Hydrolysis Method
李佳蔚 1曾珊 1杨君扬 1陈忠琴 2谭明堂 2高加龙 2郑惠娜 2苏伟艺 3曹文红2
作者信息
- 1. 广东海洋大学 食品科技学院,广东 湛江 524088
- 2. 广东海洋大学 食品科技学院,广东 湛江 524088;国家贝类加工技术研发分中心,广东省水产品加工与安全重点实验室,广东 湛江 524088
- 3. 广东省佛山市顺德区市场监管许可技术审查中心,广东 佛山 528399
- 折叠
摘要
以香港牡蛎为原料,通过酶解法制备富锌牡蛎酶解产物并对其相对分子量图谱和氨基酸组成进行分析.经过蛋白酶筛选、超声预处理后应用单因素试验、正交试验以及时间曲线优化酶解工艺,提高酶解产物的水解度和生物锌释放量.采用超声功率500 W、超声时间10 min进行预处理,选择动物蛋白酶进行酶解.最佳酶解工艺为加酶量1.6%、酶解时间4 h、酶解温度50℃、料液比1∶4、pH 6.5,得到牡蛎酶解产物水解度为49.581%.酶解产物的锌含量(以干基计)为680.831 mg/kg.牡蛎肽相对分子量小于1 000 Da的组分达到81.87%,富含酸性氨基酸谷氨酸和天冬氨酸.香港牡蛎酶解产物中含有丰富的小分子肽,氨基酸组成较完善,锌含量较高,可为后续富锌产品的开发奠定基础.
Abstract
With Crassostrea hongkongensis as the raw material,the zinc-rich oyster enzymatic hydrolysate is prepared by enzymatic hydrolysis method,and its relative molecular weight chromatogram and amino acid composition are analyzed.Through protease screening and ultrasound pretreatment,single factor test,orthogonal test and time curves are used to optimize the enzymatic hydrolysis process,so as to improve the hydrolysis degree of enzymatic hydrolysate and the release amount of biological zinc.Using ultrasonic power of 500 W and ultrasonic time of 10 min for pretreatment,animal protease is selected for enzymatic hydrolysis.The optimal enzymatic hydrolysis process is as follows:the enzyme addition amount is 1.6%,enzymatic hydrolysis time is 4 h,enzymatic hydrolysis temperature is 50℃,solid-liquid ratio is 1∶4 and pH is 6.5.The hydrolysis degree of the obtained oyster enzymatic hydrolysate is 49.581%.The zinc content of the enzymatic hydrolysate(on dry basis)is 680.831 mg/kg.The components of oyster peptides with relative molecular weight less than 1 000 Da reach 81.87%,which are rich in acid amino acids,namely glutamic acid and aspartic acid.The enzymatic hydrolysate of Crassostrea hongkongensis contains abundant small-molecular peptides,with a relatively complete amino acid composition and high zinc content,which can lay a foundation for the development of zinc-rich products in the future.
关键词
香港牡蛎/富锌/牡蛎肽/超声辅助酶解/低分子量Key words
Crassostrea hongkongensis/zinc-rich/oyster peptide/ultrasound-assisted enzymatic hydrolysis/low molecular weight引用本文复制引用
基金项目
湛江市科技计划(2022A05038)
国家重点研发计划(2018YFD0901105)
国家贝类产业技术体系建设项目(CARS-49)
出版年
2024
NETL
NSTL
万方数据