Staphylococcus cohnii subsp.cohnii HT-26 Isolated from Instant Meat Products and Screening of Its Rapid Detection Molecular Marker
Staphylococcus cohnii is a kind of skin symbiotic bacterium,but it can also cause inflammation and diseases under certain conditions.It is commonly distributed in the environments such as humans,livestock and food.In this study,a strain of Staphylococcus cohnii HT-26 is isolated from commercially available instant meat products.After analysis,it is identified that the strain belongs to Staphylococcus cohnii subsp.cohnii(SCC).Due to the high similarity of genomic sequences between SCC and its close-related species,there are certain difficulties in the rapid and spectific detection of SCC.In this study,based on comparative genomics analysis,four SCC specific sequences are found,and a pair of efficient specific detection primers is screened and verified,which can achieve SCC detection at the minimum concentration of 27 CFU/mL and can be effectively applied in instant meat products,with the minimum detection limit of 115 CFU/g.Implementing rapid and specific SCC detection on instant meat products or other foods can strengthen the prevention and monitoring of microbial contamination in seasonings and further enhance food safety assurance.
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