Establishment of Fingerprint of Pomegranate Peel Extracts and Determination of Content of Index Components
To establish a high performance liquid chromatography for the fingerprint of pomegranate peel extracts and determination of the content of index components,and to provide references for quality control of pomegranate peel extracts,in this paper,the fingerprint of pomegranate peel extracts is established by high performance liquid chromatography,and the quality difference of ten batches of pomegranate peel extracts is analyzed by similarity evaluation combined with chemometrics method.At the same time,with punicalagin and gallogen as the index components,the content determination method is established.The results show that a total of 28 common peaks are identified in the established fingerprints of ten batches of pomegranate peel extracts,among which,No.8 peak is α-punicalagin,No.13 peak is β-punicalagin,and No.28 peak is gallogen.The similarity of pomegranate peel extracts from batches S2,S4 and S10 is<0.9.The ten batches of pomegranate peel extracts are divided into five categories by cluster analysis,and through VIP value screening,punicalagin and gallogen are identified as the main differential index components.The content of punicalagin and gallogen is measured to be 28.80～288.90 mg/g and 6.12～17.73 mg/g respectively.The fingerprint and content determination method established in the research can provide references for quality control and evaluation of pomegranate peel extracts.
pomegranate peel extractfingerprintcontent determinationquality research
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NSTL
万方数据
