Safety Assessment of Levilactobacillus brevis M-10 Isolated from Sour Porridge in Northwestern Shanxi
A strain of Levilactobacillus brevis M-10 is isolated from the fermentation broth of sour porridge in northwestern Shanxi.It is found that it has various probiotic characteristics such as acid resistance,bile salt resistance,antioxidation,anti-bacteria,lowering cholesterol.In order to better apply this strain,in vitro and in vivo safety is evaluated,including hemolytic activity detection,gelatin liquefaction activity detection,nitroreductase activity detection,indole and biogenic amine production ability detection,acute and subacute toxicity experiments in SD rats.After detection,it is found that Levilactobacillus brevis M-10 lacks hemolytic activity,cant liquify gelatin,lacks nitroreductase activity,cant produce indole or biogenic amines,including tyramine,histamine,cadaverine,putrescine and so on.This strain has no obvious adverse effects on the general physical signs and blood routine indexes of SD rats.The results indicate that Levilactobacillus brevis M-10 has good safety.This study could lay foundation for the subsequent development of Levilactobacillus brevis M-10 as fermentation agent or probiotic.
sour porridge in northwestern ShanxiLevilactobacillus brevishemolytic activitygelatin liquefaction activitynitroreductase activitydetection of indoledetection of biogenic aminesacute and subacute toxicity experiments
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