Optimization of Extraction Process of Polysaccharides from Hawthorn and Yam by Response Surface Methodology and Study on Their Antioxidant Activities
In this study,the process of ultrasound-assisted hot water extraction of hawthorn polysaccharides and yam polysaccharides is optimized,and their monosaccharide composition and antioxidant activity are investigated.On the basis of single factor test,with ultrasonic time,solid-liquid ratio and extraction temperature as the factors and polysaccharide extraction yield as the response value,the model is established and analyzed by response surface method.It is found that the optimal parameters of extraction process of polysaccharides from hawthorn are ultrasonic time of 28 min,solid-liquid ratio of 1∶24.8(g/mL),extraction temperature of 71.5 ℃ and extraction time of 120 min,under these conditions,the extraction yield is 49.09％.The optimal parameters of extraction process of polysaccharides from yam are ultrasonic time of 42 min,solid-liquid ratio of 1∶30(g/mL),extraction temperature of 70.0 ℃ and extraction time of 180 min,under these conditions,the extraction yield is 12.81％.The monosaccharide composition and proportion of polysaccharides from hawthorn and yam are determined by ion chromatography.In vitro antioxidant test shows that the scavenging rates of hawthorn polysaccharides on DPPH free radicals,ABTS free radicals and hydroxyl free radicals are 80.58％,97.61％,68.4％respectively,showing stronger free radical scavenging capacity.
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