Separation and Purification of Chlorogenic Acid from Blueberry Leaf Polyphenols by Medium Pressure Liquid Chromatography
In this study,with blueberry leaves as the test materials,ultrasonic-assisted cellulase method is used to obtain crude polyphenol extracts,and then the crude extracts are separated and purificated using medium pressure liquid chromatography technology,and the effects of different mobile phases,sample volumes and mobile phase flow rates on the yield of chlorogenic acid are investigated.The results show that the optimal preparation conditions are acetonitrile-aqueous solution as the mobile phase,the sample volume of 6 mL,and the mobile phase flow rate of 10 mL/min.Under these conditions,the yield of chlorogenic acid is 21.39％.Through high-performance liquid chromatography analysis,it is found that the purity of the obtained chlorogenic acid can reach 94.98％.Chlorogenic acid from blueberry leaves can be quickly and efficiently separated by this method.This method has provided an effective means for studying and applying the active components in blueberry leaves.
blueberry leaf polyphenolsmedium pressure liquid chromatographyseparation and purificationchlorogenic acid
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