Study on Extraction Process of Complex Enzyme Method,Chemical Composition and Antioxidant Activity of Polysaccharide from Tetranephris brasiliensis
With Tetranephris brasiliensis powder as the research object,the extraction process of complex enzyme method,chemical composition and antioxidant activity of Tetranephris brasiliensis polysaccharide(TBP)are studied.TBP is extracted by complex enzyme method.The effects of enzymatic hydrolysis pH,the addition amount of complex enzymes(the ratio of cellulase to papain of 1∶1),enzymatic hydrolysis temperature and enzymatic hydrolysis time on the extraction rate of TBP are investigated,and response surface model is used to optimize the extraction process of TBP.The content of chemical components,monosaccharide composition,functional group structure and in vitro antioxidant activity of TBP are analyzed and studied.The results show that the optimal extraction process parameters of TBP by complex enzyme method are enzymatic hydrolysis pH of 6.1,addition amount of complex enzymes of 5.35％,enzymatic hydrolysis temperature of 53.1 ℃ and enzymatic hydrolysis time of 84.2 min.The extraction rate of TBP can reach(8.86±0.13)％under the optimal extraction conditions.Through chemical composition analysis,it is found that the total sugar content of TBP is(57.59±1.06)％,protein content is(5.14±0.88)％,and glucuronic acid content is(3.16±0.42)％.TBP has typical characteristic peaks of carbohydrates,and it contains pyranose and sulfate groups,with a total of ten types of monosaccharides,mainly including glucose,galactose and mannose.The antioxidant activity of TBP is studied,and it is found that TBP has good scavenging capacity on ABTS radicals,superoxide anion radicals and hydroxyl radicals.This study can provide references for the extraction of Tetranephris brasiliensis polysaccharide and its application in food industry.
NETL
NSTL
万方数据
