滇产刺梨多糖的提取纯化及其结构分析
Extraction,Purification and Structure Analysis of Polysaccharides from Rosa roxburghii Tratt.Produced in Yunnan Province
杨紫焰 1李倩倩 1李自霖 1陈贵元 2张翠香1
作者信息
- 1. 大理大学基础医学院,云南大理 671000
- 2. 大理大学基础医学院,云南大理 671000;云南省昆虫生物医药研发重点实验室,云南大理 671000
- 折叠
摘要
目的:探讨刺梨多糖提取和纯化的方法.方法:采用热水提取醇沉法从刺梨果实中提取多糖,在单因素实验的基础上,以响应面实验对刺梨多糖的提取工艺进行优化.采用Cellulose DEAE-52和Sephadex G-100柱对刺 梨粗多糖进行分离纯化,Sevage法脱蛋白,D-101吸附树脂脱色素,FT-IR、HPGPC和HPLC测定分子量和单糖组成.结果:刺梨多糖是一种以Glc为主要成分的均一性酸性多糖.
Abstract
Objective:To study the extraction and purification methods of polysaccharides from Rosa roxburghii Tratt..Methods:Polysaccharides are extracted from Rosa roxburghii Tratt.fruit by hot water extraction and alcohol precipitation method.On the basis of single factor experiment,the extraction process is optimized by response surface experiment.The crude polysaccharides are separated and purified by Cellulose DEAE-52 and Sephadex G-100 columns,deproteinized by Sevage method,decolorated by D-101 adsorptivon resin,and the molcecular weight and the composition of monosaccharides are determined by FT-IR,HPGPC and HPLC.Results:Rosa roxburghii Tratt.polysaccharide is a kind of homogeneous acid polysaccharide with Glc as the main component.
关键词
刺梨多糖/提取纯化/脱蛋白/脱色/Cellulose/DEAE-52/Sephadex/G-100Key words
Rosa roxburghii Tratt.polysaccharides/extraction and purification/deproteinization/decolorization/Cellulose DEAE-52/Sephadex G-100引用本文复制引用
基金项目
国家自然科学基金项目(31860252)
云南省自然科学基金地方高校联合面上项目(202301BA070001-043)
云南省教育厅科学研究基金项目(2021J0337)
出版年
2024
NETL
NSTL
万方数据