In order to obtain high-producing adenosine strains and realize cost reduction and efficiency enhancement,a strain of adenosine-producing bacterium,Bacillus subtilis SB-37,is breeded by ARTP mutation,and single factor experiment and response surface method are used to optimize the fermentation conditions of the strain.The results show that the optimal treatment time is 30 s and the mutation fatality rate is 98.75％.Compared with the adenosine yield of the original strain,8 high-producing adenosine mutant strains are obtained,and strain Q40-2-37 has the highest adenosine yield of 6.20 g/L,which is 2.58 times that of the original strain.The optimal fermentation conditions of the strain are glucose of 99 g/L,peptone of 25 g/L,pH of 7.04,inoculation amount of 8.03％and fermentation time of 72 h.Under these conditions,the adenosine yield is 6.92 g/L.The results of this study have provided technical support for the mutation and breeding of high-producing adenosine strains and optimization of fermentation conditions.
关键词
腺苷/枯草芽孢杆菌/常压室温等离子体/诱变/发酵条件优化
Key words
adenosine/Bacillus subtilis/atmospheric and room temperature plasma/mutation/optimization of fermentation conditions
维普
万方数据