Study on Composition of Fatty Acids in Zanthoxylum bungeanum from Different Places of Origin Based on Principal Component Analysis and Cluster Analysis
In order to investigate the difference of the composition of fatty acids in Zanthoxylum bungeanum from different places of origin,with Zanthoxylum bungeanum from 22 different places of origin as the raw materials,Soxhlet extraction method is used to extract fat,potassium hydroxide methanol method is used for esterification,gas chromatography is used to determine the composition of fatty acids,and principal component analysis and cluster analysis are used for comprehensive evaluation.The results show that there are mainly 12 kinds of fatty acids in Zanthoxylum bungeanum,which are myristic acid,myristoleic acid,palmitic acid,palmitoleic acid,heptadecanoic acid,cis-10-heptadecenoic acid,stearic acid,oleic acid,linoleic acid,arachidic acid,γ-linolenic acid and α-linolenic acid.It is mainly composed of unsaturated fatty acids,with oleic acid,linoleic acid,linolenic acid and palmitoleic acid accounting for higher proportion.Saturated fatty acids are mainly palmitic acid and stearic acid.Combining the fatty acid content,principal component analysis and cluster analysis results of 22 samples of Zanthoxylum bungeanum,it is found that based on the differences of the composition of fatty acids of Zanthoxylum bungeanum from different provinces,it is capable of classifying the places of origin,but the differences of the composition of fatty acids among Hancheng and other places of origin in Shaanxi cannot be distinguished.This study has accumulated data for the differentiation of places of origin and quality level evaluation of Zanthoxylum bungeanum,and provided technical support for the formulation of relevant standards for Zanthoxylum bungeanum and Zanthoxylum bungeanum oil.