摘要
该研究对前期提取的黑果腺肋花楸发酵果渣中的粗多酚进行分离纯化.通过比较吸附和解吸效果,选择NKA-9作为试验树脂;采用响应面法优化得到的多酚最佳纯化工艺为样液pH值3.0、样液浓度2.0 mg/mL、上样流速2.0 mL/min、乙醇浓度70％和洗脱流速1.0 mL/min,在此条件下,多酚纯度由(11.71±0.20)％提高到(62.61±0.78)％,提高了 5.34倍;HPLC分析发现,多酚纯化物中含有绿原酸、金丝桃苷、芦丁、新绿原酸、原儿茶酸、阿魏酸、表儿茶素没食子酸酯、咖啡酸和槲皮素等多酚成分,其中绿原酸含量最高;多酚浓度为 55 µg/mL 时,对 DPPH·、·OH 和 ABTS+·的清除率分别为(96.06±0.45)％、(89.95±0.19)％和(99.33±0.02)％;清除自由基能力及对Fe3+的还原能力显著高于VC对照组.
Abstract
In this study,the previously extracted crude polyphenols from Aronia melanocarpa fermented fruit residue are isolated and purified.NKA-9 is selected as the test resin by comparing the adsorption and desorption effects.The optimum purification process of polyphenols obtained by response surface method is as follows:the pH value of sample solution is 3.0,the concentration of sample solution is 2.0 mg/mL,the loading velocity is 2.0 mL/min,the ethanol concentration is 70％and the elution velocity is 1.0 mL/min.Under these conditions,the purity of polyphenols increases from(11.71±0.20)％to(62.61±0.78)％,increasing by 5.34 times.Through HPLC analysis,it is found that the purified polyphenols contain chlorogenic acid,hyperin,rutin,neochlorogenic acid,protocatechic acid,ferulic acid,epicatechin gallate,caffeic acid and quercetin.The chlorogenic acid has the highest content.When the concentration of polyphenols is 55 μg/mL,the scavenging rates on DPPH·,·OH and ABTS4+·are(96.06±0.45)％,(89.95±0.19)％,(99.33±0.02)％respectively.The free radical scavenging capacity and the reducing power of Fe34 are significantly higher than those of VC control group.
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