Study on Purification of Blueberry Anthocyanins by Mixed Macroporous Resins and Their Antioxidant Activity
Purification process conditions of blueberry anthocyanins by mixed macroporous resins are studied to improve the antioxidant effect of blueberry anthocyanins.The adsorption effects of five macroporous resins such as D101,HP-20,AB-8,NKA-9 and D3520 on blueberry crude extract are compared.With adsorption rate and desorption rate as the evaluation indexes,the adsorption and desorption conditions for the separation and purification of blueberry anthocyanins by mixed resins are optimized through single factor test and orthogonal test.The antioxidant properties of blueberry anthocyanins before and after purification are analyzed,and the differences in hydroxyl free radicals(·OH)and 2,2-diphenyl-1-picrylhydrazine free radicals(DPPH·)scavenging rates of anthocyanins before and after purification are compared.The results show that D101 and HP-20 resins are mixed in a ratio of 9∶1,and the adsorption effect is better when the ratio of the mixed resins to blueberry crude extract is 1∶1.When the ultrasonic frequency is 40 kHz,the concentration of adsorption sample solution is the original sample solution,the adsorption time is 40 min,and the adsorption temperature is 20 ℃,the adsorption rate reaches 96.2％.When the loading volume is 5 mL,the loading concentration is the original sample solution,the elution velocity is 1.0 mL/min,the eluent ethanol concentration is 60％,and the eluent volume is 3 BV,the desorption rate is as high as 95.7％.The·OH and DPPH·scavenging rates of blueberry anthocyanins purified with mixed resins are higher than those before purification.The scavenging rates of anthocyanins on·OH before and after purification are 96.25％and 97.85％respectively.The scavenging rates of anthocyanins on DPPH·before and after purification are 88.35％and 93.20％respectively.The mixed resins of D101 and HP-20 have good purification effects on blueberry anthocyanins and can be used for the purification of blueberry anthocyanins.After purification,the antioxidant capacity of blueberry anthocyanins is significantly improved,which can better scavenge·OH and DPPH·,and the scavenging effect on·OH is higher than that on DPPH·.
mixed resinsblueberry anthocyaninsseparation and purificationantioxidant activity
万方数据
维普
