Optimization of Ultrafiltration Extraction Process of Capsicum Seed Protein Isolate and Characterization of Its Characteristics
As the main by-product of Capsicum processing,Capsicum seed is rich in protein and other nutrients,but it has not been effectively used.Based on this,in this study,with Yidu red Capsicum seed as the raw material,ultrafiltration assisted alkali-solution and acid-precipitation method is used to extract Capsicum seed protein isolate,and the comprehensive extraction rate,material input,physicochemical and functional characteristics of Capsicum seed protein isolate extracted by ultrafiltration assisted alkali-solution and acid-precipitation method are compared with those extracted by traditional alkali-solution and acid-precipitation method.The optimal process parameters of Capsicum seed protein isolate by traditional alkali-solution and acid-precipitation method are solid-liquid ratio of 1∶40,extraction temperature of 40 ℃,extraction time of 180 min,extraction frequency of 1 time and alkali solution pH of 10.In order to increase the extraction amount of protein,reduce the dosage of acid and alkali to regulate pH,and improve the physicochemical characteristics of protein isolate,5 kDa and 10 kDa dual-stage membrane ultrafiltration technology assisted alkali-solution and acid-precipitation method is used to obtain three ultrafiltration components,including＞10 kDa protein,5～10 kDa protein and＜5 kDa protein.Compared with traditional alkali-solution and acid-precipitation method,the extraction amount of protein increases by 5.28％and the dosage of hydrochloric acid decreases by 19.48％.The solubility,water holding capacity and emulsification stability of＞10 kDa protein significantly increase by 9.88％,78.66％,22.69％respectively compared with those extracted by traditional alkali-solution and acid-precipitation method.The results indicate that Capsicum seed protein isolate with better physicochemical characteristics could be obtained by ultrafiltration assisted alkali-solution and acid-precipitation method,which is a promising method for extracting Capsicum seed protein isolate.
Capsicum seed protein isolatealkali-solution and acid-precipitation methodultrafiltrationprocess optimizationphysicochemical and functional characteristics
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