Study on Extraction of Protein from Potato by Different Extraction Methods
In this experiment,with fresh potatoes as the raw materials,potato protein is extracted by three methods such as alcohol dissolution method,salt dissolution method,alkali dissolution method,and its purity and extraction rate are determined.The results show that the purity of potato protein extracted by alcohol dissolution method is 94.5％,the extraction rate is 4.5％,and the extraction effect is the best.SDS-PAGE electrophoresis analysis shows that the potato protein extracted by alcohol dissolution method has many subunit bands,a wide range of molecular weights,and a high content in the molecular weight range of 25～40 kDa,indicating that alcohol dissolution method could effectively extract various components of potato protein.Further research on its physicochemical properties shows that the protein extracted by alcohol dissolution method has better oil holding capacity,water holding capacity,emulsibility and emulsification stability.Hydrolysis analysis shows that potato protein extracted by alcohol dissolution method has a higher degree of hydrolysis.Therefore,among the three methods,alcohol dissolution method is a more economical extraction method to obtain protein with better properties.
potato proteinextraction methodfunctional characteristicsdegree of hydrolysis
万方数据
维普
