With Zanthoxylum bungeanum seed protein extraction rate as the evaluation index,single factor test and response surface test are used to investigate the effects of solid-liquid ratio,pH value,extraction temperature and extraction time on Zanthoxylum bungeanum seed protein extraction rate.The composition of Zanthoxylum bungeanum seed protein is explored by SDS-PAGE gel electrophoresis and amino acid analysis.The results show that the optimal extraction process conditions are solid-liquid ratio of 1∶35(g/mL),pH value of 9.0,extraction temperature of 45 ℃ and extraction time of 3.5 h.The extraction rate of Zanthoxylum bungeanum seed protein under these conditions could reach 46.54％.Zanthoxylum bungeanum seed protein consists of six major subunits,namely 54,31,25,18,10,9 kDa,among which,the content of 31 kDa protein is the most abundant.Zanthoxylum bungeanum seed protein has a complete variety of amino acids,among which,the content of Glu is the hightest of 16.37 g/100 g.In addition,the content of essential amino acids,hydrophobic amino acids and acidic amino acids in Zanthoxylum bungeanum seed protein is higher,which is 36.88,34.75,24.65 g/100 g respectively.In conclusion,Zanthoxylum bungeanum seed protein is a kind of high-quality protein resource with high nutritional value and it may have antioxidant function.
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