Optimization of Extraction Process of Active Substances from Lycium barbarum and Determination of Activity
The active components in Lycium barbarum are extracted by water extraction method,and solid-liquid ratio,enzymatic hydrolysis temperature and enzymatic hydrolysis pH are selected as the three single factors,the extraction process of polysaccharides and proteins is optimized by response surface method on the basis of single factor experimental results,and the best extraction process is obtained as follows:solid-liquid ratio is 1∶25.29,enzymatic hydrolysis temperature is 59.29 ℃,and enzymatic hydrolysis pH is 5.94.The theoretical content of polysaccharides and proteins is 79.36％,and the average content is 79.05％after three times of extraction under the optimal extraction process.It can be seen that the extraction process obtained by response surface method has high stability and reliability.The antioxidant activity of the extracts is determined,and the results show that the extracts of Lycium barbarum have a certain scavenging effect on superoxide anion radical,but the scavenging effect is not obvious;they have a good scavenging effect on hydroxyl radical,which is equivalent to VC at low concentration;in the DPPH radical scavenging experiment,their scavenging capacity is weaker than VC at low mass concentration,but gradually close to VC at high mass concentration,indicating that Lycium barbarum has strong antioxidant ability.
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