Optimization of Extraction Process of Tuna Fish Bone Peptide by Response Surface Method
Using ultra-low temperature tuna fish bone as the raw material,tuna fish bone peptide is extracted and prepared by enzymatic method,and complex protease is selected as the best enzyme for extraction.On the basis of single factor test,with enzyme addition amount,enzymatic hydrolysis temperature and enzymatic hydrolysis time as the independent variables,with the extraction rate of tuna fish bone peptide as the response value,the extraction process is optimized by response surface method based on Box-Behnken central combination principle,and the optimal enzymatic hydrolysis process conditions are determined as follows:the addition amount of complex protease is 625 U/g,the enzymatic hydrolysis time is 5.5 h and the enzymatic hydrolysis temperature is 50 ℃.Under these conditions,the extraction rate of tuna fish bone peptide is 34.58％,the oligopeptide content of the prepared tuna fish bone peptide is 91.7％,the oligopeptide with molecular weight ≤1 kDa accounts for 98.7％,the content of free amino acids of tuna fish bone peptide is 10.66 g/100 g,and the content of flavor amino acids and essential amino acids is relatively high,which is 26.4％and 48.1％respectively.It is a kind of marine biological resource with great exploration value.
tuna fish bone peptideextraction processresponse surface optimization test
万方数据
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