Analysis of Differences of Volatile Oil Components and Content of Turmeric from Different Resources Based on GC-MS
To compare the similarities and differences of volatile oil components of turmeric from different resources,the volatile oils of 24 batches of turmeric samples are extracted by steam distillation method,and the chemical components of each chromatographic peak are separated and identified by gas chromatography-mass spectrometry(GC-MS).Hierarchical cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least square-discriminant analysis(OPLS-DA)are used to analyze the components of 24 batches of volatile oils and screen the differential metabolites.The results show that the extraction rate of volatile oils of 24 batches of turmeric is 0.30％～1.10％,and 63 components are detected,mainly are turmerones,with the relative content more than 50％,followed by terpenes,with low content and complex composition.The GC-MS fingerprint of volatile oils of turmeric is established with similarity of 0.911～0.997,and seven common peaks are identified.HCA and PCA divide 21 samples into groups A and B,and 19 differential metabolites are screened by OPLS-DA.Cluster heat map analysis shows that the characteristic components of group A are turmerones such as aromatic curcumone,α-curcumone and β-curcumone,and the characteristic components of group B are 16 terpenes such as gingerene,β-bisabolene and β-sesquiphellandrene.The results show that the quality of volatile oils of turmeric from different places of origin is different.The content of turmeric is directly related to the color of turmeric section.The higher the content,the darker the color of turmeric.This study has provided scientific basis for the identification and quality evaluation of turmeric germplasm resources in the future by comparing 24 batches of volatile oil components of turmeric from different sources and their differential substances,and six excellent germplasm with high content of volatile oils and turmerones are selected,which could be used to guide the breeding of new varieties of turmeric.
万方数据
维普
