中国调味品2024,Vol.49Issue(9) :186-191.DOI:10.3969/j.issn.1000-9973.2024.09.028

超声波辅助提取野山竹多酚工艺优化及抗氧化活性研究

Optimization of Ultrasonic-Assisted Extraction Process of Polyphenols from Garcinia oblongifolia and Study on Antioxidant Activity

唐婷范 李雪松 黄英凤 田玉红 徐紫薇 程昊
中国调味品2024,Vol.49Issue(9) :186-191.DOI:10.3969/j.issn.1000-9973.2024.09.028

超声波辅助提取野山竹多酚工艺优化及抗氧化活性研究

Optimization of Ultrasonic-Assisted Extraction Process of Polyphenols from Garcinia oblongifolia and Study on Antioxidant Activity

唐婷范 1李雪松 1黄英凤 1田玉红 1徐紫薇 1程昊1
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作者信息

  • 1. 广西科技大学生物与化学工程学院,广西糖资源绿色加工重点实验室,广西柳州螺蛳粉工程技术研究中心,广西柳州 545006
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摘要

以野山竹果壳为原料,进行野山竹多酚提取工艺优化及其抗氧化活性的研究,建立以单宁酸溶液为标准品,采用福林酚法和分光光度法测定野山竹多酚含量的定量分析方法.结果显示:在提取时间35 min、超声温度70 ℃、提取pH 5、料液比1∶65(g/mL)、超声功率400 W的条件下多酚提取得率可高达0.73%.野山竹多酚对1,1-二苯基-2-苦基肼自由基(DPPH·)和羟基自由基(·OH)均有较强的清除效果,最大清除率分别为84.2%和82.0%.

Abstract

With the shell of Garcinia oblongifolia as the raw material,the extraction process of polyphenols from Garcinia oblongifolia is optimized and their antioxidant activity is investigated,and a quantitative analytical method is established to determine the polyphenol content of Garcinia oblongifolia with tannic acid solution as the standard by Folin-Ciocalteu method and spectrophotometry.The results show that under the conditions of extraction time of 35 min,ultrasonic temperature of 70 ℃,extraction pH of 5,solid-liquid ratio of 1∶65(g/mL)and ultrasonic power of 400 W,the polyphenol extraction yield could be as high as 0.73%.The Garcinia oblongifolia polyphenols show strong scavenging effects on both 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)and hydroxyl radical(·OH),with the maximum scavenging rates of 84.2%and 82.0%respectively.

关键词

野山竹/多酚/提取工艺/抗氧化性/清除率

Key words

Garcinia oblongifolia/polyphenol/extraction process/antioxidant activity/scavenging rates

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基金项目

广西自然科学基金面上项目(2021GXNSFAA220067)

广西研究生教育创新计划项目(YCSW204511)

国家自然科学基金项目(22268012)

出版年

2024
中国调味品
全国调味品科技情报中心站

中国调味品

CSTPCD北大核心
影响因子:0.541
ISSN:1000-9973
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