Simultaneous Determination of Residual Amount of Five Lipid Soluble Pigments and Five Poppy Husk Alkaloids in Seasonings by UHPLC-MS/MS Method
A high-throughput analysis method is established for the simultaneous determination of residual amount of five lipid soluble pigments such as Sudan Red Ⅰ～Ⅳ and Para Red,as well as five poppy husk alkaloids such as papaverine,morphine,codeine,thebaine and narcotine in seasonings using QuEChERS EMR(Enhanced Matrix Removal)-Lipid purification combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Papaverine,thebaine,narcotine and five lipid soluble pigments show a good linear relationship within the range of 1～100 ng/mL,the correlation coefficients are all greater than 0.99,and the limit of quantification(LOQ)is 1.0 μg/kg.Morphine and codeine show a good linear relationship within the range of 8～100 ng/mL,the correlation coefficients are both greater than 0.99,and the limit of quantification(LOQ)is 5.0 μg/kg.The average recovery rates of each compound range from 81.2％to 104.5％,with the relative standard deviations between 2.9％and 11.4％(n=6).The method is simple,sensitive,stable,high-throughput and low-cost,and it is suitable for the detection and quality control requirements of various seasonings such as spice flavored oil,hotpot seasoning and dipping sauce,and Malatang seasoning.
万方数据
维普
