摘要
建立了 一种经QuEChERS EMR(Enhanced Matrix Removal)-Lipid净化结合超高效液相色谱-串联质谱(UHPLC-MS/MS)同时测定调味品中苏丹红Ⅰ～Ⅳ、对位红5种脂溶性色素和罂粟碱、吗啡、可待因、蒂巴因、那可丁 5种罂粟壳生物碱残留量的高通量分析方法.罂粟碱、蒂巴因、那可丁和5种脂溶性色素在1～100 ng/mL范围内线性关系良好,相关系数均大于0.99,定量限(LOQ)为1.0 μg/kg;吗啡、可待因在8～100 ng/mL范围内线性关系良好,相关系数均大于0.99,定量限(LOQ)为5.0 μg/kg;各化合物平均回收率为81.2％～104.5％,相对标准偏差为2.9％～11.4％(n=6).该方法简便、灵敏、稳定、通量高、成本低,适用于香辛料调味油、火锅底料及蘸料、麻辣烫底料等多种调味品的检测质控需求.
Abstract
A high-throughput analysis method is established for the simultaneous determination of residual amount of five lipid soluble pigments such as Sudan Red Ⅰ～Ⅳ and Para Red,as well as five poppy husk alkaloids such as papaverine,morphine,codeine,thebaine and narcotine in seasonings using QuEChERS EMR(Enhanced Matrix Removal)-Lipid purification combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Papaverine,thebaine,narcotine and five lipid soluble pigments show a good linear relationship within the range of 1～100 ng/mL,the correlation coefficients are all greater than 0.99,and the limit of quantification(LOQ)is 1.0 μg/kg.Morphine and codeine show a good linear relationship within the range of 8～100 ng/mL,the correlation coefficients are both greater than 0.99,and the limit of quantification(LOQ)is 5.0 μg/kg.The average recovery rates of each compound range from 81.2％to 104.5％,with the relative standard deviations between 2.9％and 11.4％(n=6).The method is simple,sensitive,stable,high-throughput and low-cost,and it is suitable for the detection and quality control requirements of various seasonings such as spice flavored oil,hotpot seasoning and dipping sauce,and Malatang seasoning.
基金项目
山东省重大科技创新工程项目(2019JZZY011014)
潍坊市科技发展计划项目(2021GX020)
维普
万方数据