Optimization of Ultrasonic-Assisted Extraction Process of Polyphenols from Leaves of Elaeagnus moorcroftii Wall.by Response Surface Methodology and Study on Their Antioxidant and Anti-Glycation Capacity
In the process of promoting circular economy,the extraction of polyphenols from Elaeagnus moorcroftii Wall.leaves and their application potential are focused on in this study.The extraction process of polyphenols from the leaves of Elaeagnus moorcroftii Wall.is optimized by ultrasonic-assisted extraction technology,and their antioxidant and anti-glycation abilities are evaluated comprehensively.The optimal extraction conditions are determined as ultrasonic time of 20.00 min,solid-liquid ratio of 1∶53.40(g/mL),ultrasonic temperature of 69.70 ℃,ultrasonic power of 400 W.Under such conditions,the extraction amount of polyphenols is(28.24±0.46)mg/g.Antioxidant tests show that the polyphenols have stronger scavenging abilities on DPPH·,·OH and ABTS+·,and their IC50 values are 0.112,0.110,0.146 mg/mL respectively.Under the BSA-GLC simulated reaction system,the inhibition rate of glycosylation significantly increases to(44.64±7.20)％.The research results show that the polyphenols extracted by ultrasonic-assisted extraction technology have significant antioxidant and anti-glycation abilities in vitro,and their abilities increase with the increase of concentration of polyphenols from Elaeagnus moorcroftii Wall.leaves,which indicates that they could be used as potential natural food additives,and has provided a new direction for the comprehensive utilization of by-products of Elaeagnus moorcroftii Wall..
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