Determination of Trivalent Chromium and Hexavalent Chromium in Zanthoxylum bungeanum by Ion Chromatography-Inductively Coupled Plasma Mass Spectrometry
In order to analyze the trivalent chromium and hexavalent chromium in Zanthoxylum bungeanum,ion chromatography-inductively coupled plasma mass spectrometry is used to determine their content. Methodological investigation,content determination and in vitro simulation of the conversion of trivalent chromium and hexavalent chromium under gastric acid conditions are conducted. The results show that the method can separate trivalent chromium and hexavalent chromium within 3 min,and the peak shape is good. The method has a good linear relationship (the mass concentration of standard curve is 0.5～20 μg/mL,r>0.999). The quantification limits of trivalent chromium and hexavalent chromium are 0.06 mg/kg and 0.07 mg/kg respectively. The spiked recovery rates of trivalent chromium and hexavalent chromium in Zanthoxylum bungeanum,Zanthoxylum schinifolium and Zanthoxylum armatum (with spiked amount of 0.2,0.5,1.0 mg/kg respectively) are 80％～100％,and the RSDs are all less than 10％,which indicates that the applicability,accuracy and precision of the method are good. There is a great difference in total chromium content in Zanthoxylum bungeanum,Zanthoxylum schinifolium and Zanthoxylum armatum. Hexavalent chromium is not detected in 30 batches of Zanthoxylum bungeanum. Trivalent chromium in Zanthoxylum bungeanum accounts for 1％～10％ of the total chromium content. Under the conditions of 37 ℃,pH 2～3 hydrochloric acid (gastric acid),the conversion rates of hexavalent chromium in the gastric acid group and Zanthoxylum bungeanum group both significantly increase with the increase of time. With the same addition amount and the same conversion time,the conversion rate of hexavalent chromium in Zanthoxylum bungeanum group is significantly higher than that in the gastric acid group,indicating that Zanthoxylum bungeanum is conducive to the conversion of hexavalent chromium to trivalent chromium. This paper has provided a theoretical basis and methodological support for the analysis of chromium forms in Zanthoxylum bungeanum.
Zanthoxylum bungeanumtrivalent chromiumhexavalent chromiumion chromatographyinductively coupled plasma mass spectrometry
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维普
