目的:探究大鼠力竭运动时皮层-基底神经节通路振荡电活动变化特征及其振荡电活动与运动能力的相关性。方法:采用在体多通道神经电信号记录技术，记录安静，第1、3和7天大鼠力竭运动时大鼠皮层(M1)和基底神经节(Str、GPe、SNr和STN)的局部场电位(local field potentials，LFPs);采用功率谱密度(power spectral density，PSD)探究力竭运动时各核团不同频段振荡电活动的功率谱特征;利用同步似然分析(synchronization likelihood，SL)探究皮层-基底神经节网络的功能连接变化;采用Pearson相关分析探究β频段(12～30 Hz)PSD值和SL系数与大鼠力竭运动时长的相关性。结果:大鼠运动力竭即刻与安静时相比，1)振幅分析发现，LFPs平均振幅值均显著升高(P<0。01);2)功率谱密度分析发现，皮层和基底神经节各核团在β频段的PSD值均不同程度显著升高(P<0。05);3)同步似然分析发现，间接通路的路径Str-GPe、GPe-STN、GPe-SNr、STN-SNr和超直接通路M1-STN的β频段的平均SL系数均明显增高(P<0。05)，且升高幅值都是GPe-STN最大、M1-STN最小，而直接通路(Str-SNr)在各频段中无显著差异(P>0。05)，经24 h休息可恢复到静息状态水平(P>0。05);4)Pearson相关分析发现，除Str外，其他核团的β频段的PSD值与大鼠力竭运动时长呈负相关;间接通路路径GPe-STN(R=-0。67，P<0。01)和STN-SNr(R=-0。56，P<0。01)与大鼠力竭运动时长呈负相关，而超直接通路M1-STN和直接通路Str-SNr不相关(P>0。05)。结论:力竭运动时皮层-基底神经节通路的β振荡增强，超直接和间接通路功能性连接显著增强，这一现象可能与运动能力的抑制相关，表明短期的β振荡增强并不一定是病理性的，而可能是神经系统对力竭运动的一种适应性反应。
Study on the Oscillatory Electrical Activity Characteristics of the Cortico-Basal Ganglia Pathway During Exhaustive Exercise in Rats
Objective:This study aims to investigate the oscillatory electrical activity characteris-tics in the cortical-basal ganglia pathway and their correlation with motor abilities during ex-haustive exercise of rats. Methods:A multichannel system was employed for in vivo neural sig-nal recording,synchronously capturing local field potentials (LFPs) from the cortical-basal gan-glia pathway (M1,Str,GPe,SNr and STN) immediately following 1,3 and 7 days of exercise-induced exhaustion and after 24 hours of recovery. LFP amplitude and power spectral density (PSD) were analyzed to determine the coding characteristics of oscillatory activity associated with 1,3 and 7 days of exercise-induced fatigue. Synchronization likelihood (SL) analysis was utilized to identify functional connectivity imbalances among the pathways linking the cortex and basal ganglia. Pearson correlation analysis estimated the association between PSD values and SL coefficients of the β-band (from 12 Hz to 30 Hz) and the duration of exercise-induced fa-tigue over the periods of 1,3 and 7 days. Results:Compared to sedentary controls,exhaustive exercise led to significant findings:1) an increase in the mean amplitude of LFPs (P<0.01);2) a significant rise in PSD values at the β-band in cortical and basal ganglia areas immediately post-exercise,which returned to baseline after 24 hours of recovery;3) an increase in mean SL coefficients at the β-band in the indirect (Str-GPe,GPe-STN,GPe-SNr,STN-SNr) and hyperdi-rect (M1-STN) pathways,with the largest amplitude observed in GPe-STN and the smallest in M1-STN (P<0.05);Str-SNr in the direct pathway showed no significant changes (P>0.05). 4) Pearson correlation analysis revealed significant negative correlations between the duration of exercise-induced fatigue and SL coefficients in GPe-STN (R=-0.67,P<0.01) and STN-SNr (R=-0.56,P<0.01). In contrast,M1-SNr in the hyperdirect and Str-SNr in the direct pathways showed no significant correlations (P>0.05). Conclusion:This study reveals that during exhaus-tive exercise,there is an enhancement of β oscillations within the cortico-basal ganglia path-ways and a disruption in the functional connectivity of the hyperdirect and indirect pathways. This phenomenon may be related to the inhibition of motor ability,suggesting that short-term enhancement of β oscillations is not necessarily pathological but may represent an adaptive re-sponse of the nervous system to exhaustive exercise.
exhaustive exercisecortical-basal gangliaoscillatory activitylocal field poten-tialsynchronization likelihood analysis
NETL
NSTL
万方数据
