一种新型胃转流手术治疗2型糖尿病GK大鼠模型的建立
Establishment of a new gastric bypass animal-model with GK rats
孙庆磊 1王宇 2张新国 3高宏凯 3赵靓4
作者信息
- 1. 山东省滨州医学院附属医院普通外科
- 2. 解放军总医院普通外科
- 3. 100039,北京,武警总医院普通外科
- 4. 山东省滨州市人民医院外科
- 折叠
摘要
目的 建立保留全胃不同区段小肠转流的胃转流手术(GBP)治疗2型糖尿病(T2DM)的动物模型.方法 3月龄自发性T2DM GK大鼠40只,随机分为4组(每组10只)实施保留全胃的GBP:Ⅰ组为对照组,Ⅱ组为十二指肠转流组,Ⅲ组为空肠转流组,Ⅳ组为回肠转流组.分别于术前1 d和术后1、4、8周检测比较空腹血糖及测量大鼠体质量.结果 全组大鼠手术存活率95%,Ⅳ组有2只大鼠于术后第1天死亡.4组大鼠术前空腹血糖依次为(13.80±1.86)mmol/L、(12.02±1.97)mmol/L、(13.42±1.66)mmol/L和(14.32±2.82)mmol/L;转流术后4周,Ⅱ、Ⅲ、Ⅳ组大鼠空腹血糖分别降至(6.36±0.50)mmol/L、(5.96±0.53)mmol/L和(5.18±0.49)mmol/L;与术前比较,差异有统计学意义(P<0.01);与对照组同期[(14.16±2.26)mmol/L]比较,差异也有统计学意义(P<0.01);但各转流术组间比较,差异无统计学意义(P>0.05).4组大鼠术前体质量依次为(253.6±9.37)g、(268.2±7.95)g、(253.0±6.20)g和(262.0±13.47)g,术后4周则分别为(367.0±23.70)g、(384.8±16.12)g、(323.0±16.40)g、和(185.8±11.56)g,除Ⅳ组大鼠体质量明显降低(P<0.01)外,其他组均较术前明显增加(P<0.05).结论 保留全胃的GBP对GK大鼠有明显降糖作用,但降糖效果并不随转流肠袢长度的增加而增加,且与大鼠的体质量增减无关.
Abstract
Objective To establish a new gastric bypass animal-model with Goto-Kakizaki rats whose different parts of the small intestine were bypassed while stomach was not bypassed. Methods Forty male 3-month-old GK rats were randomly divided into 4 groups: group Ⅰ (sham operation), group Ⅱ (duodenum bypassed), group Ⅲ (jejunum bypassed), group Ⅳ(ileum bypassed). Fasting plasma glucose was measured before operation and the 1st, 4th,and 8th week after operation in all the rats, the body weight of all the rats were measured simultaneously. Results The survival rate of opration for the rats was 95%. Two rats in group Ⅳ died on the first day after operation. The mean fasting plasma glucose concentration of the rats in group Ⅱ, Ⅲ, Ⅳ declined obviously 4 weeks after gastric bypass [group Ⅱ (12.02±1.97) vs (6.36±0.50) mmol/L, group Ⅲ (13.42±1.66) vs (5.96± 0.53) mmol/L, group Ⅳ (14.32±2.82) vs (5.18±0.49) mmol/L, all P<0.01], but there were no significant differences among the gastric bypassed groups. The weight of rats in group Ⅰ, Ⅱ, Ⅲ increased obviously after gastric bypass [group Ⅰ (253.6±9.37) vs (367.0±23.70) g, group Ⅱ (268.2± 7.95) vs (384.8±16.12) g, group Ⅲ (253.0±6.20) vs (323.0±16.40) g, all P<0.05] except the rats in group Ⅳ [(262.0±13.47) vs (185.8±11.56) g]. Conclusions The mean fasting plasma glucose concentration of the GK rats decreases obviously after gastric bypass through different parts of small intestine. The fasting plasma glucose concentration is not associated with the length of small intestine and body weight.
关键词
糖尿病,2型/胃旁路术/模型,动物Key words
Diabetes mellitus, type 2/Gastric bypass/Disease models, animal引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据