首页|Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

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Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding bio-chemical microenvironment,playing a crucial role in pathological processes such as tumor invasion and metastasis.How-ever,conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration.Fortunately,the further development of microfluidic technology has provided solutions for the study of such questions.We utilize microfluidic chip to build a random collagen fiber microenvironment(RFM)model and an oriented collagen fiber microenvironment(OFM)model that resemble early stage and late stage breast cancer microenvironments,respectively.By combining cell culture,biochemical concentration gradient construction,and microscopic imaging techniques,we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells.The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model,with significant differences observed.Furthermore,we establish concen-tration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model,with stronger inhibition on the high concentration side compared to the low concentration side.However,the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak.These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished.The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development,but also serve as a tool for the initial measurement of drug screening.

microfluidic chiprandom collagen fiber microenvironment(RFM)oriented collagen fiber mi-croenvironment(OFM)cancer cell migration

姚静如、李国强、姚喜耀、周连杰、叶志凯、刘艳平、郑栋天、唐婷、宋克纳、陈果、刘雳宇

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Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials,College of Physics,Chongqing University,Chongqing 401331,China

Chongqing Key Laboratory for Resource Utilization of Heavy Metal Wastewater,Aquatic Ecosystems in the Three Gorges Reservoir Region of Chongqing Observation and Research Station,Chongqing University of Arts and Sciences,Yongchuan 402160,China

Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China

Department of Biomedical Engineering,Chongqing University of Posts and Telecommunications,Chongqing 400065,China

College of Medical Technology and Engineering,Henan University of Science and Technology,Henan 471023,China

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国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金Fundamental and Advanced Research Program of Chongqing重庆市自然科学基金Science and Technology Research Program of Chongqing Municipal Education CommissionScientific Research Fund of Chongqing University of Arts and SciencesScientific Research Fund of Chongqing University of Arts and SciencesCollege Students'Innovation and Entrepreneurship Training Program of Chongqing Municipal

119740661217404112104134T235000712347178cstc2019jcyjmsxmX0477CSTB2022NSCQ-MSX1260KJQN202301333R2023HH03P2022HH05S202310642002

2024

10.1088/1674-1056/ad334c

中国物理B(英文版)
中国物理学会和中国科学院物理研究所

中国物理B(英文版)

CSTPCDEI
影响因子：0.995
ISSN：1674-1056
年,卷(期)：2024.33(5)
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