Self-Assembly of Bacteria in Alternating-Current Electric Fields

李艳冉 ¹宗奕吾 ¹张红 ²张静超 ³冯春迎 ⁴乔建军 ⁵宋浩 ⁵赵坤⁶

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作者信息

1. Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China
2. Petrochemical Research Institute of Petrochina Co.,Ltd.,Beijing 102206,China
3. Center for Medical Genetics,Sichuan Provincial People's Hospital,University of Electronic Science and Technology of China,Chengdu 611731,China
4. Jiangsu Key Laboratory of Sericultural and Animal Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang 212100,China;Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,Sericultural Scientific Research Center,Chinese Academy of Agricultural Sciences,Zhenjiang 212100,China
5. Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China
6. The Sichuan Provincial Key Laboratory for Human Disease Gene Study and The Institute of Laboratory Medicine,Sichuan Provincial People's Hospital,University of Electronic Science and Technology of China,Chengdu 610054,China;Institute of Fundamental and Frontier Sciences,University of Electronic Science and Technology of China,Chengdu 610054,China
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Abstract

Self-assembly of bacteria in electric fields is a promising route to fabricate biomaterials with reversible and specific structures.However,due to relatively less studies,our understanding of the self-assembly of bacteria in electric fields is still incomplete.Particularly,how different bacterial species behave differently in their field-mediated self-assembly behavior remains to bo disclosed.In this study,we choose four bacterial species,including Shewanella oneidensis,Pseudomonas aeruginosa,Bacillus subtilis and Lactococcus lactis as model systems,and investigate their self-assembly behavior in alternating-current(AC)electric fields for both diluted and concen-trated suspensions.The phase diagrams in the plane of applied field strength vs frequency are obtained.The results show that in diluted suspensions,a transition sequence of isotropic-paranematic-string-columnar phases is observed in all strains as the field strength increases.Details of the assembled structures are quantitatively differentiated among different strains.In concentrated suspensions,besides the isotropic and paranematic phases,a higher ordered phase with interdigitating rectangular crystal domains(OIR)and an ordered phase with smectic A liquid crystal domains are observed for S.oneidensis and P.aeruginosa,respectively.Our findings shed new light on fabricating potential biomaterials by assembling cells of appropriately chosen bacterial species that have desired surface properties under AC electric fields.

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基金项目

National Key R&D Program of China(2023YFC3402401)

National Natural Science Foundation of China(12374206)

出版年

2024

中国物理快报(英文版)

中国科学院物理研究所,中国物理学会

中国物理快报(英文版)

CSTPCDCSCDEI

影响因子：0.515

ISSN：0256-307X

参考文献量38

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