Objective To explore the application value of microfiltration membrane separation technology in microbial testing process.Methods A total of 86 microbial samples received by Ji'nan Center for Disease Control and Prevention from January 2022 to June 2023 were used as the research subjects.From each sample to be tested,10 mL were taken for microbiological examination by plate counting method,and 10 mL were taken for bacterial cells by microfiltration membrane separation technology for microbiological examination.The microbial detection rate,microbial detection reporting time and viable bacterial recovery rate of the two test methods were compared and observed to evaluate the test effect.Results A total of 47 positive samples were detected using both methods,of which 45 were detected using microfiltration membrane separation technology,accounting for 95.74%,and 47 were detected using plate counting method,accounting for 100%.There was no statistically significant difference in the positive detection rate between the two methods in the initial examination of 86 samples(P>0.05).Based on the retest results,the microfiltration membrane separation technology missed 2 cases in the initial examination.There was no statistically significant difference in the retest positive rate and missed rate between the two methods(P>0.05).The average cultivation time of microfiltration membrane separation technology was(20.24±3.17)h,which was shorter than that of plate counting method(32.75±4.36)h,and the difference was statistically significant(P<0.05).The random sampling recovery rate experiment showed that the positive bacterial recovery rates of the microfiltration membrane separation technology were 87.69%,87.50%,79.07%,67.86%,and 83.51%,respectively,which were generally higher than those of the plate counting method(72.01%,71.38%,64.19%,47.32%and 65.64%),the difference was statistically significant(P<0.05).Conclusion The positive detection rate of using microfiltration membrane separation technology in microbial testing is equivalent to that of plate counting method,but the former has a shorter cultivation time,an earlier reporting time for microbial testing,a higher recovery rate of positive bacteria,and a better effect than plate counting method.
关键词
微生物检验/微滤技术/膜分离/细菌培养/微生物检出率/出报告时间
Key words
microbial testing/microfiltration technology/membrane separation/bacterial culture/microbial detection rate/reporting time
维普
万方数据