Uncertainty Evaluation of Total Colony Count Testing in Sushi
Objective To analyze the sources of uncertainty in measurement of the total bacterial count of 20 sushi samples,calculate the uncertainty of Class A and class B,evaluate the uncertainty of measurement,and determine the confidence interval.Methods Select 20 commercially available sushi from April to August 2023 for analysis.According to the requirements of"Evaluation and expression of uncertainty in measurement",the food safety were calculated according to"National Standard for food safety,microbiological examination,determination of total colony count"and related statistical methods.Results The uncertainty of class A was 0.044 5,class B was 0.014 7,the uncertainty of synthetic standard was 0.046 9,and the uncertainty of expanded was 0.097 8.Conclusion The laboratory needs a stable environment to carry out microbial culture,which can effectively guarantee the accuracy and precision of test results,the uncertainty evaluation of the total number of food colony is an important means to ensure the safety and quality of food.
uncertaintytotal colony countclass A uncertaintyclass B uncertaintycombined standard uncertaintyexpanded uncertainty