目的 通过转录组测序，探讨毗邻锌指结构域的溴结构域蛋白 1A(bromodomain adjacent to zinc finger domain 1A，BAZ1A)在肝癌和子宫颈癌中促癌机制的共同点。方法 在肝癌和子宫颈癌转录组测序结果中使用"DESeq2"包进行筛选肝癌组差异表达基因(different expression genes，DEGs)和子宫颈癌组DEGs。肝癌组DEGs与子宫颈癌组DEGs取交集获得共有DEGs。通过基因本体论(gene ontology，GO)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes，KEGG)对以上 3 组DEGs相关的功能及通路进行分析。采用STRING数据库对上述 3 组DEGs进行蛋白质互作(protein-protein interaction，PPI)网络的富集分析，并使用Cytoscape软件筛选出各组DEGs中的核心基因。结果 得到肝癌组DEGs为 294 个，子宫颈癌组DEGs为 5 662 个，共有DEGs为 170 个。肝癌组DEGs、子宫颈癌组DEGs和共有DEGs都富集到的KEGG通路包括细胞外基质-受体相互作用通路、焦点黏附通路。肝癌组DEGs和子宫颈癌组DEGs都显著富集的GO条目包括细胞运动、生物黏附。PPI网络分析得到肝癌组核心基因为H4 簇状组蛋白 5(H4 clustered histone 5，H4C5)、H4 簇状组蛋白 14(H4 clustered histone 14，H4C14)，子宫颈癌组核心基因分化簇 44(cluster of differentiation，CD44)、激太原 1(kininogen 1，KNG1)、圆盘大MAGUK支架蛋白 4(discs large MAGUK scaffold protein 4，DLG4)，共有组核心基因为H2A簇状组蛋白 18(H2A clustered histone 18，H2AC18)。结论 在肝癌和子宫颈癌中，BAZ1A都可以通过影响癌细胞运动、迁移的能力，发挥促癌作用。
Analysis of the Co-Carcinogenic Mechanism of BAZ1A in Hepatocellular Carcinoma and Cervical Cancer
Objective Through transcriptome sequencing,the common mechanism of bromodomain adjacent to zinc finger domain 1A(BAZ1A)in promoting cancer in hepatocellular carcinoma and cervical cancer was explored.Methods The"DESeq2"package was used to screen different expression genes(DEGs)in the hepatocellular carcinoma group and the cervical cancer group in the transcriptome sequencing results of hepatocellular carcinoma cancer and cervical cancer.The DEGs of hepatocellular carcinoma group and cervical cancer group were intersected to obtain common DEGs.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to analyze the functions and pathways related to the above three groups of DEGs.The protein-protein interaction(PPI)network enrichment analysis of the above three groups of DEGs was performed by STRING database,and the core genes in each group of DEGs were screened by Cytoscape software.Results A total of 294 DEGs were obtained for the hepatocellular carcinoma group,5 662 DEGs for the cervical cancer group,and 170 common DEGs.KEGG pathways enriched in DEGs of hepatocellular carcinoma group,DEGs of cervical cancer group and common DEGs included ECM-receptor interaction pathway and focal adhesion pathway.GO items significantly enriched in DEGs of both hepatocellular carcinoma group and cervical cancer group included locomotion and biological adhesion.PPI network analyses showed that the core genes of hepatocellular carcinoma group were H4 clustered histone 5(H4C5)and H4 clustered histone 14(H4C14),the core genes of cervical cancer group were cluster of differentiation(CD44),kininogen 1(KNG1)and discs large MAGUK scaffold protein 4(DLG4),and the core gene of the common group was H2A clustered histone 18(H2AC18).Conclusion In both hepatocellular carcinoma and cervical cancer,BAZ1A can play a role in promoting cancer by affecting the ability of cancer cell movement and migration.
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